母源性丙烯酰胺暴露对断乳期大鼠额叶皮质GAP-43和BDNF表达的影响  

Influence of maternal exposure on the expression of GAP-43 and BDNF in the cerebral frontal cortex of weaning rats

作　　者：李细霞[1] 袁丽鹏 肖春苟[1] 刘靖[2] 蒋威[1] 邹锦慧[1] 张霞[1] 

机构地区：[1]肇庆医学高等专科学校,肇庆526020 [2]广东药科大学,广州510006

出　　处：《神经解剖学杂志》2017年第1期36-40,共5页Chinese Journal of Neuroanatomy

基　　金：广东省医学科学基金项目(A201618);广东省中医药局中医药常规科研项目(20162156);肇庆市科技创新指导项目(2014E184);肇庆市医学高等专科学校青年科技基金(zqyq16-001)

摘　　要：目的:探讨母源性丙烯酰胺(acrylamide,ACR)暴露对断乳期大鼠额叶皮质生长相关蛋白(GAP-43)和脑源性神经营养因子(BDNF)表达的影响。方法:SD孕鼠随机分为4组,怀孕第6 d起对照组和实验组分别给予双蒸水和50,100,200μg/ml ACR饮水,直至仔鼠出生21 d,染毒结束时对断乳期大鼠称重。免疫组织化学检测额叶皮质GAP-43和BDNF的表达。结果:与对照组相比,实验各组断乳期大鼠体重呈浓度依赖性下降,且组间差异明显(P<0.05)。与对照组相比,ACR实验各组GAP-43表达显著降低(P<0.05),而低、中剂量组之间差异不显著(P>0.05),高剂量组与低、中剂量组相比,表达显著减少(P<0.05);与对照组相比,ACR实验组各组BDNF表达显著减少(P<0.05),中、高剂量组与低剂量组相比,BDNF表达减少(P<0.05),而中、高剂量组之间差异不显著(P>0.05)。结论:母源性ACR染毒可能通过下调额叶皮质神经元GAP-43和BD)NF表达,抑制轴突延伸或扰乱神经元发育的内环境稳态,进而影响额叶皮质的发育。Objective;To explore the influence of maternal acrylamide exposure on the expression of growth associated protein（GAP-43） and brain derived neurotrophic factors（BDNF） in the cerebral frontal cortex of weaning rats.Methods：The SD pregnant rats were randomly divided into 4 groups and provided with doubly evaporated water in control groups and50,100,200 μg/ml ACR solution via drinking water in experimental groups respectively from gestational day 6 to postnatal day 21 and weighed the weaning rats at the end of the experiment.The expression of GAP-43 and BDNF was observed in the weaning rats frontal cortex neurons by immunohistochemistry.Results：Compared with the control group,the body weight of weaning rats in the ACR experimental groups decreasedsignificantly in a dose dependent manner（P〈0.05）,and the difference between ACR experimental groups was also significant P〈0.05）.Compared with the control group,the expression of GAP-43 in each ACR experimental group decreased significantly（P〈0.05）,while the expression of GAP-43 between the low-dose group and mid-dose group had no statistical significance（P〉0.05）,the expression of CAP-43 in high-dose group decreased significantly than that of the low-dose group and mid-dose group（P〈0.05）;Compared with the control group,the expression of BDNF in each ACR experimental group were lower than that of the control group,this index of the mid-dose group and high-dose group is much more lower than that of the low-dose group（P〈0.05）,while there were no significant difference between the mid-dose group and high-dose group（P〈0.05）.Conclusion：Maternal acrylamide exposure might inhibit axonal plasticity and interfere with homeostasis by decreasing the expression of CAP-43 and BDNF,which then may affect the development of frontal cortex.

关 键 词：丙烯酰胺 神经发育毒性 GAP-43 BDNF 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]