槲皮素对小鼠前额叶皮质锥体神经元的放电抑制作用研究  

The inhibition effect of quercetin on the action potential of the pyramidal cells in prefrontal cortex in mice

作　　者：任可可[1] 刘慧浪 刘霞[1] 白占涛[1] 武胜昔[3] 王文挺[3] 

机构地区：[1]延安大学生命科学学院,延安716000 [2]云南省药物研究所云南白药集团创新研发中心云南省中药和民族药新药创制企业重点实验室,昆明650111 [3]第四军医大学基础部神经生物学教研室暨脑科学协同创新中心,西安710032

出　　处：《神经解剖学杂志》2017年第1期41-46,共6页Chinese Journal of Neuroanatomy

基　　金：科技部国际科技合作专项(2011DFA32560);国家自然科学基金(81371498)

摘　　要：目的:探讨槲皮素对小鼠前额叶皮质(prefrontal cortex,PFC)锥体神经元动作电位的效应。方法:成年C57小鼠或Thy1-GCaMP 3.0小鼠,制作含PFC的冠状脑片。分成正常脑脊液孵育组(对照组)和槲皮素孵育组(50μmol/L),给予高钾(5 mmoL/L KCl)人工脑脊液灌流两组脑片记录GCaMP荧光信号,全细胞膜片钳记录两组PFC锥体神经元放电,碘化丙啶(propidium iodide,PI)染色检测神经元活性。结果:5 mmol/L KCl人工脑脊液可以明显引起对照组GCaMP脑片PFC锥体神经元荧光信号增强,而对槲皮素组无明显作用。全细胞膜片钳记录显示槲皮素组神经元能够产生动作电位。但与对照组相比,产生放电所需刺激强度增加,连续放电能力降低。PI染色显示槲皮素组神经元死亡数目减少。结论:槲皮素孵育可以通过抑制小鼠前额叶皮质锥体神经元产生动作电位的能力,从而提高神经元在体外环境的生存能力。其具体的离子通道机制及细胞信号通路研究需进一步的深入研究。Objective;To explore the effect of quercetin on the action potential of the pyramidal cells in prefrontal cortex（PFC） in mice.Methods：The coronal brain slices including PFC were made from adult C57 mice or Thy1-GCaMP3.0 mice.The slices were cut in hemi-slices and incubated in two groups;Control group with normal ACSF and quercetin group with 50（μmol/L quercetin adding into ACSF.The calcium fluorescent signals changes were induced by5 mmol/L KC1 in ACSF in the slices from GCaMP mice.The action potentials of pyramidal cells were generated by current injection with whole cell patch clamp.PI（Propidium Iodide） staining was used for detecting the dead cells in the slices.Results：GCaMP signals changes in quercetin group were smaller than control group when the FPC slices were treated with 5 mmol/L KC1.The threshold current for generating action potential in quercetin group also was bigger than control group with whole cell patch clamp recording.And the numbers of dead cells stained by propidium iodide in quercetin group also were smaller than it in the control group.Conclusion;Quercetin could inhibit the generation of action potential in the pyramidal cells of PFC,which might be one of possible mechanisms for keeping neurons survival ability from in vitro environment.The further experiments need to be done to explore the detailed mechanisms,such as ion channels targets and signaling pathways.

关 键 词：槲皮素 前额叶皮质 神经元 动作电位 GCaMP 小鼠 

分 类 号：R285.5[医药卫生—中药学]