保护地黄瓜根内和土壤中丛枝菌根真菌和深色有隔内生真菌多样性研究（英文）  

Diversity of arbuscular mycorrhizal fungi and dark septate endophytes in the greenhouse cucumber roots and soil

作　　者：胡玉金 高春梅[1] 刘杏忠[2] 刘润进[1] 

机构地区：[1]青岛农业大学菌根生物技术研究所,山东青岛266109 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101

出　　处：《菌物学报》2017年第2期164-176,共13页Mycosystema

基　　金：Supported by the National Natural Science Foundation of China(31470101,31272210);the Basic Research Project of Qingdao Government[12‐1‐4‐5‐(14)‐jch]

摘　　要：对于野生植物根内定殖的丛枝菌根真菌(AMF)和暗隔内生真菌(DSE)多样性及其生态功能现已进行了众多的调查研究。然而,对于同时定殖于栽培作物同一根系的这两种真菌的物种多样性和功能了解甚少。本研究旨在采用传统的形态学方法和PCR‐DGGE技术探究保护地栽培的黄瓜Cucumis sativus Linn.根内AMF和DSE的物种多样性。PCR‐DGGE结果显示共有7种AMF,包括Funneliformis mosseae,Glomus fasciculatum,Glomus indicum,Scutellospora dipurpurescens,Gigaspora margarita以及2个未培养的Archaeospora;而以黄瓜植株根段作为接种物加富培养后,依据其所产生的孢子形态特征进行分类鉴定,则只分离获得3种,即F.mosseae,G.indicum和Gi.Margarita;同时,采用常规分离纯化的方法从黄瓜根内分离得到DSE 6个菌株,其中1株经分子生物学鉴定为Phoma leveillei。从保护地根区土壤中分离AMF孢子并通过形态学分类鉴定,获得了9属20种。研究结果表明,Glomus是保护地栽培黄瓜根系内的优势属,针对数量,相对于传统形态鉴定技术,分子技术可以检测到根内更多的AMF。The diversity and its ecological functions of arbuscular mycorrhizal fungi （AMF） and dark septate endophytes （DSE） on wild plants have been well documented. However, the species of AMF and DSE and their function simultaneously colonizing on the same root system of cultivated crops are less investigated. The purpose of the present study was to monitor AMF and DSE in the roots of cucumber （Cucumis sativus Linn.） and soils in the greenhouse condition by using traditional morphological methods and nested polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）. For the AMF on cucumber roots, seven species including Glomus fasciculatum, Glomus indicum, Funneliformis mosseae, Scutellospora dipurpurescens, Gigaspora margarita and two uncultured species of Archaeospora were detected with PCR-DGGE, while only F. mosseae, G. indicum and Gi margarita were detected based on the morphological identification of spores collected from the root trap culture. Meanwhile, six solates of DSE were isolated from the cucumber root segments and one of them was identified as Phoma leveillei with PCR-DGGE Twenty species in nine genera of AMF were isolated and identified based on spores extracted in the cucumber root zone soil. Glomus was the dominant genus in cucumber roots under greenhouse conditions. More AMF species in cucumber roots were detected by molecular techniques in comparison with the species detected by the trap culture and the spore morphological methods .

关 键 词：丛枝菌根真菌 深色有隔内生真菌 农业保护地土壤 球囊霉属 茎点霉 黄瓜根系 

分 类 号：S626[农业科学—园艺学] S642.2S182