绵羊血清蛋白双向凝胶电泳技术的建立  

Establishment of Two- dimensional Gel Electrophoresis Technique for Sheep Serum

作　　者：张译元[1] 唐红[1] 郭延华[1] 王新华[1] 王立民[1] 周平[1] 

机构地区：[1]新疆农垦科学院畜牧兽医研究所/省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子832000

出　　处：《新疆农业科学》2017年第1期190-196,共7页Xinjiang Agricultural Sciences

基　　金：兵团院士资金专项(2015AG018);新疆农垦科学院青年科学基金(YQJ201502)~~

摘　　要：【目的】建立和优化绵羊血清蛋白双向电泳体系(2-DE)。【方法】从样品提取及处理、IPG胶条的选择、SDS-PAGE浓度的选择等多个方面对双向电泳分离条件进行比较。【结果】采用10%TCA/丙酮沉淀法处理血清蛋白质,18 cm p H 4~7的IPG胶条2.5 mg上样量,10%SDS-PAGE,获得的2-DE图谱效果较好。【结论】获得良好的绵羊血清蛋白双向电泳图谱,建立并优化了绵羊蛋白质组学的双向电泳技术体系,为转基因绵羊生物安全性评价提供了重要的技术条件。[ Objective] To establish two- dimensional gel eleetrophoresis methods （2 -DE） for sheep serum. [Method]The experimental conditions of 2 -DE were optimized, and various extraction and process methods, different pH IPG strips, different concentration of SDS - PAGE were studied. [ Result] The results showed that the 10% tca/ acetone precipitation method was used to deal with the serum protein, pH 4 - 7 IPG, 2.5 rag, 18 era, 10% sds- page, and a good 2 -DE map was obtained. [ Conclusion]This study got a good IPG maps for sheep serum protein. We have successfully established a 2 - DE technique for sheep serum proteome, which can provide important technical information for evaluation of transgenic sheep biological safety.

关 键 词：绵羊 血清 蛋白质组 双向电泳 

分 类 号：S826[农业科学—畜牧学] S188[农业科学—畜牧兽医]