富血小板血浆结合松质骨移植治疗腕舟骨骨不连的实验研究  

Platelet Rich Plasma Combined with Cancellous Bone Graft in the Treatment of Scaphoid Nonunion Effect

作　　者：刘星[1] 田利军[1] 邓志刚[1] 郭宇松[1] 张三冰 高金宝[2] 

机构地区：[1]石家庄市第三医院创伤2科,050011 [2]石家庄市第三医院手足外科,050011

出　　处：《医学研究杂志》2017年第2期83-85,共3页Journal of Medical Research

基　　金：河北省医学科学研究基金资助项目(20160777)

摘　　要：目的通过实验研究探讨富血小板血浆结合松质骨移植治疗腕舟骨骨不连的效果。方法健康新西兰大白兔36只,随机分为A、B两组,每组18只,两组建立腕舟骨骨不连动物模型后,A组为腕舟骨骨不连处植入松质骨治疗,B组为舟骨骨不连处植入松质结合PRP治疗,术后8周观察动物一般状况与骨愈合情况。结果术后A组骨基质可见且钙盐沉积不明显,B组骨不连区能够看到诸多新生骨,但新生骨量较A组低;术后8周B组实验侧修复性新骨形成均多于A组,对比差异有统计学意义(P<0.05)。术后8周观察组的软骨总体形态与微形态评分分别为1.74±0.43分和3.20±1.74分,对照组分别为3.61±0.56分和7.42±1.24分,观察组明显少于对照组(P<0.05)。两组胶原含量在术后都呈现明显上升的趋势,术后8周观察组与对照组的胶原含量分别为0.24±0.03ng/L和0.17±0.06ng/L,观察组明显高于对照组(P<0.05)。结论富血小板血浆结合松质骨移植治疗腕舟骨骨不连对骨的再生与修复有明显的促进作用,应用比较方便,有比较好的应用价值。Objective To investigate the experiment effects of platelet rich plasma combined with cancellous bone graft in the treatment of scaphoid nonunion. Methods Thirty-six healthy New Zealand white rabbits were selected and were randomly and equally divided for group A and group B with 18 rats in each group, the two groups were established scaphoid bone nonunion animal model, group A were implantation treated with cancellous bone at scaphoid bone, group B were implantation treated with navicular bone combined wiht cancellous PrP therapy, postoperative 8 weeks were to observe the general state of animals and bone healing. Results The postoperative bone in group A were not even area visible large amounts of new bone, group B were showed that the bone matrix and a small amount of calcium salt deposition, but the new bone was low than the group A; The experimental side repair of bone formation of postoperative 8 weeks in group B were higher than that in group A, the differences were statistical significance（P〈0.05）. The total cartilage morphology and micro morphology scores of the group B were 1.740.43 points and 3.201.74 points respectively, and the group A were 3.610.56 points and 7.421.24 points respectively, the group B was significantly less than that of the group A（P〈0.05）. The collagen content in the two groups were showed significant upward trend, The postoperative 8 weeks in the group B and the group A were 0.240.03ng/L and 0.170.06ng/L, the group B was significantly higher than the group A（P〈0.05）. Conclusion Rich platelet rich plasma combined with cancellous bone transplantation in the treatment of carpal scaphoid bone nonunion of bone regeneration and repair has significant role in promoting, the application is more convenient that has better application values.

关 键 词：富血小板血浆 松质骨 腕舟骨骨不连 软骨 胶原含量 

分 类 号：R6[医药卫生—外科学]