FlcA在巴西固氮螺菌Sp7中对细菌细胞壁厚度的影响  

Effect of Flc A in Azospirillum brasilense Sp7 on thickness of cell wall

作　　者：张媛媛[1] 刘帅[1] 崔佳彬 王威[1] 侯星生[1] 王桂琴[1] 

机构地区：[1]山西医科大学微生物学和免疫学教研室,山西太原030001

出　　处：《中国生物制品学杂志》2017年第2期146-149,共4页Chinese Journal of Biologicals

基　　金：山西省留学回国人员科技活动择优资助项目【2014】95号;2012年度山西医科大学校博士启动资金资助项目【2013】4号

摘　　要：目的评价Flc A在巴西固氮螺菌Sp7对细菌细胞壁厚度的影响。方法将野生型巴西固氮螺菌Sp7、flc A敲除株Sp7-flc AΔ及Tn5插入突变株Sp72001,分别在NB培养基及营养缺乏的絮凝培养基中培养,于透射电镜下观察细胞形态,测量细胞壁厚度,检测不同营养条件下Flc A对细菌外观和细胞壁厚度的影响。结果野生型巴西固氮螺菌Sp7、flc A敲除株Sp7-flc AΔ及Tn5插入突变株Sp72001在NB培养基中的细胞壁厚度的中位数分别为(34.13±12.47)、(29.94±9.25)、(34.90±14.88)nm,差异无统计学意义(P>0.05);而在絮凝培养基中培养的细胞壁厚度分别为(75.03±17.67)、(27.00±12.17)、(42.22±15.54)nm,两两比较,差异均有统计学意义(P<0.001);同时,每种菌株用两种不同培养基培养的细胞壁厚度差异有统计学意义(P<0.05)。结论在絮凝条件下,敲除flc A的细菌细胞壁比野生型菌株的细胞壁明显变薄,Flc A可能参与了细菌细胞壁的发育。Objective To evaluate the effect of Flc A in Azospirillum brasilense Sp7 on the thickness of cell wall.Methods Wild type A. brasilense strain Sp7, Sp7-flc AΔ strain with Flc A gene deleted and Sp72001 strain with Tn5 gene inserted were cultured in nutrient broth（NB）medium and flocculation medium separately, observed for cell morphology by transmission electron microscopy, and measured for thickness of cell wall, based on which the effects of Flc A on bacterial appearance and thickness of cell wall were evaluated. Results The thicknesses of cell walls in Sp7, Sp7-flc AΔ and Sp72001 strains in NB medium were（34. 13 ± 12. 47）,（29. 94 ± 9. 25） and（34. 90 ± 14. 88） nm respectively, which showed no significant difference（P〈0. 05）. However, in flocculation medium, the thicknesses were（75. 03 ± 17. 67）,（27. 00 ± 12. 17）and（42. 22 ± 15. 54）nm respectively, which showed significant difference（P〈0. 001）. Meanwhile,the thicknesses of cell walls of the same strain in two media showed significant difference（P〈0. 05）. Conclusion The cell wall in Sp7-flc AΔ strain in flocculation medium was significantly thinner than that in Sp7 strain, indicating that Flc A was involved in cell wall development.

关 键 词：FLC A 巴西固氮螺菌 细胞壁 厚度 絮凝培养基 

分 类 号：S144.5[农业科学—肥料学]