NGF和CgA对RWPE-1细胞内钙离子浓度变化的影响及其在慢性前列腺炎中的作用  

Effect of NGF and CgA on the intracellular calcium ion concentration of RWPE-1 cells and the role of NGF and CgA in chronic prostatitis

作　　者：丁华洋 樊松[1,2] 张力[1,2] 郝宗耀[1,2] 周骏[1,2] 张翼飞[1,2] 梁朝朝[1,2] 

机构地区：[1]安徽医科大学第一附属医院泌尿外科,合肥230022 [2]安徽医科大学泌尿外科研究所,合肥230032

出　　处：《安徽医科大学学报》2017年第2期173-180,共8页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81170698;81400757);安徽省自然科学基金(编号:1508085QH171);卫生部国家临床重点专科建设项目(2012)

摘　　要：目的探讨神经生长因子(NGF)和嗜铬颗粒蛋白A(CgA)处理后前列腺上皮细胞系RWPE-1细胞内钙离子浓度([Ca^(2+)]_i)的差异及NGF和CgA在慢性前列腺炎(CP)发病中的作用。方法不同浓度的NGF和CgA(50、200 ng/ml)刺激RWPE-1细胞,用激光共聚焦显微镜观察不同浓度的NGF和CgA刺激后细胞内[Ca^(2+)]_i变化,加入TRPV1受体拮抗剂后继续观察细胞内[Ca^(2+)]_i变化。然后,将正常细胞外液换成D-PBS,再加入NGF和CgA观察细胞内[Ca^(2+)]_i变化。结果 50 ng/ml NGF和CgA刺激细胞后,细胞内[Ca^(2+)]_i未见明显变化。200 ng/ml NGF和CgA刺激细胞后,细胞内[Ca^(2+)]_i明显增加,加入TRPV1受体拮抗剂后,细胞内[Ca^(2+)]_i未见明显变化。将正常细胞外液换成D-PBS,用NGF和CgA刺激后,细胞内[Ca^(2+)]_i未见明显改变。结论CP的发生可能是通过分泌和释放NGF和CgA,引起前列腺上皮细胞膜TRPV1通道开放,促使细胞内钙离子浓度增加,导致前列腺上皮分泌功能下降所致。Objective To explore difference of intracellular calcium ion （ [ Ca2 ＋ ]i ） concentration of nerve growth factor（NGF） and chromogranin A（CgA） treated prostate epithelial cell line RWPE-1 cells and evaluate the role of NGF and CgA in chronic prostatitis （CP）. Methods Using different concentrations of NGF and CgA （50 ng/ml and 200 ng/ml） to stimulate RWPE-1 cells, the difference of intraeellular [ Ca2＋ ]i concentration was observed by using Laser Scanning Confocal Microscopy. The difference of intracellular[ Ca2＋]i concentration was observed after infusing NGF and CgA mixed with TRPV1 receptor antagonist. When the extracellular solution was changed to D- PBS, the intracellular [ Ca2 ＋ ] i concentration was observed after NGF and CgA infusion. Results Change in intra- cellular [ Ca2 ＋ ] i concentration did not occur by infusing 50 ng/ml NGF and CgA. Intracellular [ Ca2 ＋]i concentra- tion significantly increased by infusing 200 ng/ml NGF and CgA. Intracellular [ Ca2 ＋] i concentration did not change after infusion of NGF and CgA mixed with TRPV1 receptor antagonist. Even when the extracellular solution was changed to D-PBS, the intracellular [ Ca2 ＋ ] i concentration did not change after NGF and CgA infusion. Con- clusion NGF and CgA might participate in the mechanism of CP and cause the opening of TRPV1 of prostate epi- thelial cell membrane. Then, the intracellular [ Ca2＋]i concentration increase. In the end, prostate epithelial secretion decline.

关 键 词：前列腺炎 神经生长因子 嗜铬颗粒蛋白A RWPE-1细胞 钙离子 

分 类 号：R697.33[医药卫生—泌尿科学]