FGF10对鸡胚尿囊膜血管形成的影响  

Influence of FGF10 on the angiogenesis of chorioallantoic membrane vessels

作　　者：刘芳[1] 李刚[1] 张丝雨[1] 张小涵 

机构地区：[1]南昌大学医学部基础医学院细胞生物学教研室,南昌330031

出　　处：《安徽医科大学学报》2017年第2期208-210,共3页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:31460259)

摘　　要：目的研究纤维母细胞生长因子FGF10对鸡胚尿囊膜血管生长作用,探讨FGF10对血管形成的影响。方法应用胶原蛋白共培养方法,将FGF10(100 ng/ml)单独或与FGF受体拮抗剂SU5402(20μmol/L)作用于鸡胚尿囊膜血管内皮细胞组织块,观察FGF10对新生血管形成的影响。结果与空白对照组比较,FGF10组新生血管形成面积显著增加(P<0.001)。SU5402可抑制FGF10的作用,与空白对照组比较,其新生血管形成面积显著下降(P<0.001)。结论FGF10能促进鸡胚尿囊膜新生血管的形成。Objective To study the effect of FGF10 on the angiogenesis of chicken chorioallantoic membrane （CAM） vessels. Methods By using collagen co-culture method, FGF10 （ 100 ng/ml） alone or with its antagonist SU5402 （20μmol/L） were acted on the endothelial vessel explants, and then the in vitro angiogenesis experiment was carried out. Results Compared with the control groups, FGF10 promoted the area of vessel outgrowth （ P 〈 0. 001 ）. However, SU5402 suppressed the effect of FGF10 ; compared with blank control groups, the area of new- born vessels decreased significantly （P 〈 0. 001 ）. Conclusion FGF10 has the function of enhancing the formation of new CAM vessels.

关 键 词：FGF10 SU5402 血管形成 

分 类 号：R331[医药卫生—人体生理学]