八角枫根中1个新的生物碱及其细胞毒活性研究  

A new cytotoxic alkaloid from roots of Alangium chinense

作　　者：邢欢欢[1] 周堃[1] 杨艳[1] 周玲[1] 董伟[1] 王月德 马航赢 周敏[1] 叶艳青[1] 胡秋芬[1] 

机构地区：[1]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650500

出　　处：《中国中药杂志》2017年第2期303-306,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(21362044;21032085);云南省高校科技创新团队项目(IRTSTYN 2014-11);2016年云南民族大学研究生创新基金科研项目(2016YJCXS13)

摘　　要：对八角枫Alangium chinense根的化学成分进行研究,运用硅胶、凝胶、MCI-gel树脂及RP-HPLC等多种色谱技术分离纯化,并根据理化性质和波谱数据鉴定化合物的结构,同时用MTT法测定化合物的细胞毒活性。从八角枫根的90%乙醇提取物中分离鉴定了3个生物碱类化合物(1~3),其中化合物1为新化合物,命名为8-羟基-3-羟甲基-6,9-二甲基-7H-苯并[de]异喹啉-7-酮,其对NB4,A-549,SHSY5Y,PC-3,MCF-7的IC50分别为4.2,3.5,5.7,2.8,3.9μmol·L-1,该化合物具一定的细胞毒活性。We have carried out the chemical investigation on the roots of Alangium chinense. The chemical constituents from the roots of A. chinense were isolated and purified by various chromatographic techniques,such as silica gel,MCI-Gel resin,Sephadex LH-20 and high performance liquid chromatography. As a result,three alkaloids（ 1-3） were isolated from 90 % Et OH extracts of the roots of this plant. Their structures were elucidated by physical-chemical properties and spectral data. Among them,compound 1 is a new compound,determined as 8-hydroxy-3-hydroxymethyl-6,9-dimethyl-7 H-benzo[de]isoquinolin-7-one. Cytotoxicity of the compounds was evaluated by the MTT method. Compound 1 displayed cytotoxicity against NB4,A-549,SHSY5 Y,PC-3 and MCF-7 cell lines with IC50 values of 4. 2,3. 5,5. 7,2. 8 and 3. 9 μmol· L- 1,respectively.

关 键 词：八角枫 生物碱 细胞毒活性 

分 类 号：R284[医药卫生—中药学]