甜樱桃砧木PcDHN1的克隆及其对非生物胁迫的响应  

Cloning of a Dehydrin Gene PcDHN1 and Its Response to Abiotic Stresses in Sweet Cherry Rootstock

作　　者：徐丽[1] 陈新[1] 宗晓娟[1] 魏海蓉[1] 张力思[1] 刘庆忠[1] 

机构地区：[1]山东省果树研究所/山东省果树生物技术育种重点实验室,山东泰安271000

出　　处：《核农学报》2017年第1期14-20,共7页Journal of Nuclear Agricultural Sciences

基　　金：山东省农业科学院青年科研基金项目(2016YQN25);山东省农业良种工程项目(鲁科农字[2014]96);山东省现代农业产业技术体系果品产业创新团队(SDAIT-06-04)

摘　　要：为明确甜樱桃砧木脱水素基因特征及其在干旱、高盐和低温胁迫过程中的表达模式,以甜樱桃砧木Y1为材料,利用RT-PCR和RACE技术克隆了甜樱桃砧木脱水素基因Pc DHN1,利用生物信息学分析其编码蛋白特征,并采用qRT-PCR分析该基因的表达模式。序列分析显示,甜樱桃砧木脱水素基因Pc DHN1的c DNA全长893 bp,编码225个氨基酸,推测编码蛋白分子量约为23.98 k D,理论等电点为8.65。氨基酸序列分析显示,Pc DHN1含有2个Y-片段、1个S-片段和2个K-片段,具有植物脱水素蛋白的特征性结构,属于YnSK2型脱水素。表达特性分析显示,Pc DHN1在干旱、高盐和低温胁迫条件下受胁迫诱导而上调表达,但对低温胁迫响应比较迟缓,说明该基因可能参与了甜樱桃砧木对干旱和高盐胁迫的耐受调节过程。本研究结果为甜樱桃砧木抗逆基因工程提供了一定的参考。Abiotic stresses are major factors which significantly affecting the growth and yield of fruits. To explicit the characteristics of a dehydrin gene and its response to drought,high-salt and cold,RT-PCR and RACE technologies were used for cloning of Pc DHN1 and bioinformatic methods were applied for characterizing the dehydrin gene and its deduced protein. The gene expression patterns were analyzed by using the real time qRT-PCR. A sweet cherry rootstock dehydrin gene Pc DHN1 was cloned and sequencing analysis showed that the c DNA of the dehydrin gene is 893 bp in length,and encodes 225 amino acids with a predicted molecular weight of about 23. 98 k D and p I of 8. 65. Amino acid sequence analysis indicated that Pc DHN1 possessed typical characters of LEA family and was sorted into YnSK2 subfamily of dehydrins. The expression analysis revealed that Pc DHN1 was induced by drought-,salt-and chilling-stresses,while insensitive to the chilling-stress. It was deduced that Pc DHN1 might play important roles in stress tolerance of drought and high-salt. These results provided references for stress-resistant genetic engineering breeding of sweet cherry rootstocks.

关 键 词：甜樱桃砧木 脱水素 非生物胁迫 表达分析 

分 类 号：S662.5[农业科学—果树学]