黄芩苷对白癜风小鼠模型的作用机制研究  

Effect of baicalin on vitiligo mice induced by monobenzone

作　　者：祝逸平[1] 金嵘[1] 王遂泉[1] 许爱娥[1] 

机构地区：[1]浙江中医药大学附属杭州市第三人民医院皮肤科

出　　处：《中国临床药理学与治疗学》2017年第1期27-32,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省自然科学基金(LQ16H290001;LQ15H290006);国家自然科学基金(81071294;81541084);市科委重大专项创新项目(20122513A02);国家临床重点专科建设项目

摘　　要：目的:探讨黄芩苷对莫诺苯宗诱导的白癜风小鼠模型发生发展的影响及其机制。方法:C57BL/6小鼠共40只分为4组,即阴性对照组、模型组、卤米松组及5%黄芩苷组,每组10只。应用40%莫诺苯宗乳膏诱导C57BL/6小鼠脱色,建立白癜风小鼠模型;进行5%黄芩苷对白癜风小鼠的疗效观察及其机制研究等;连续给药50 d,通过肉眼观察小鼠的毛发脱色,反射共聚焦显微镜(RCM)观察皮肤的黑色素和黑素细胞,免疫荧光检测CD_8^+T细胞。选取与白癜风相关的5个基因(CXCL9,CXCL10,CXCR3,RAB27A,PI3K)进行荧光实时定量PCR检测。结果:模型组小鼠在用药部位及非用药部位有毛发脱色现象。卤米松组和5%黄芩苷组小鼠脱色减少,较模型组发生率明显降低、脱色出现时间明显延迟,脱色面积减小,且局部淋巴细胞和CD_8^+T细胞浸润较模型组明显减少。荧光实时定量PCR检测提示卤米松组和5%黄芩苷组CXCL9、CXCL10、CXCR3和RAB27A表达较模型组明显降低,而PI3K则升高明显。卤米松组和5%黄芩苷组之间无明显差异,但卤米松组用药30 d后,用药部位出现皮肤萎缩等不良反应。结论:卤米松组和5%黄芩苷组均具有治疗效果,黄芩苷组更安全有效,可为临床白癜风的治疗提供参考。AIM： To study the effect of baicalin on vitiligo mice induced by monobenzone. METH- ODS： 40 C57BL/6 mice were randomly divided into four groups （n = 10 ）, i.e. the negative control group, the model group, the halometasone group and 5% baicalin group. 40% monobenzone cream was applied on C57BL/6 mice to induce vitiligo model. 5 % baicalin was used to observe its efficacy on vitiligo and possible mechanism. After treatment for 50 days, hair decolorizing was observed with na- ked eye, melanin and melanocytes were observed by reflectance confocal microscopy （RCM） , and CDs T cells were tested by immunofluorescence detection. 5 vitiligo-related genes （CXCL9, CXCL10, CX- CR3, RAB27A, PI3K） were detected by real-time fluorescence quantitative PCR. RESULTS：Mice in model group showed depigmentation at the monoben- zone application and non-application sites. The halo- metasone group and 5% baicalin group manifested less, delayed, smaller decolorization with fewer in- filtrated lymphocytes and CDs T cells compared with the model group. Expressions of CXCLg, CXCL10, CXCR3 and RAB27A detected by Fluorescence real- time quantitative PCR in the halometasone group and 5% baicalin group was significantly lower than that of the model group, while PI3K increased signifi- cantly. No significant difference was observed be- tween the halometasone group and the 5% baicalin group, yet the halometasone group showed incidence of skin atrophy after 30 days＇treatment. CONCLUSION： Halometasone and 5% baicalin are both ef- fective for treating vitiligo, while baicalin presented with safer results, which can be referential for clinical application.

关 键 词：黄芩苷 莫诺苯宗 白癜风 

分 类 号：R965.2[医药卫生—药理学]