草除灵对甘蓝型油菜苗期生理生化及超微结构的影响  

Effects of Benazolin-ethyl on Physio-chemical and Ultrastructural Attributes of Brassica napus at Seedling Stage

作　　者：臧丽丽[1] 许玲[2] 王铭[1] 崔鹏[1] 杨素[1] 徐建祥 周伟军[1] 

机构地区：[1]浙江大学作物科学研究所,浙江杭州310058 [2]浙江理工大学生命科学学院,浙江杭州310018 [3]衢州市农科院,浙江衢州324000

出　　处：《核农学报》2017年第3期597-606,共10页Journal of Nuclear Agricultural Sciences

基　　金：农业部公益性行业科研专项(201303022);浙江省科技厅公益项目(2016C32089);江苏省现代作物生产协同创新中心

摘　　要：为探明草除灵作用的生理机制及其胁迫下不同甘蓝型油菜品种的耐性差异机制,以浙大619、浙大622为试验材料,对苗期油菜叶面喷施不同浓度的草除灵,采用生理生化分析方法、叶绿素荧光技术和超显微技术,探究草除灵对甘蓝型油菜苗期生理生化特性以及超微结构的影响。结果表明,低浓度(2.5 g·L^(-1))的草除灵对2个油菜品种的生长无显著影响,当草除灵浓度大于5.0 g·L^(-1)时,随着处理浓度的升高,油菜生长受抑制的程度逐渐增加,净光合速率、叶绿素含量和叶绿素荧光值显著下降,抗氧化酶系统的活性降低,膜脂过氧化加剧,活性氧积累增多,叶绿体、线粒体等叶肉细胞超微结构受损,且浙大619的抗草除灵胁迫能力高于较敏感品种浙大622。因此,本研究明确了草除灵对甘蓝型油菜安全性的影响,这对该除草剂的田间推广应用具有重要意义。In order to illustrate their tolerance mechanism, two leading cultivars of Brassica napus（cvs. Zheda 619 and Zheda 622） were investigated to study the effects of Benazolin-ethyl on physio-chemicat and ultrastructural attributes of Brassica napus at seedling stage, by spraying various concentrations of benazolin-ethyl to rape seedlings in terms of various physiological and biochemical measurements and microscopic analysis. The main results were as follows： low concentration（2.5 g· L-1 ） of benazolin-ethyl had no significant effect on Brassica napus cvs. Zheda 619 and Zheda 622. It was found that with the increasing of treated concentrations, the growth of Brassica napus was inhibited more when the concentration of benazolin-ethyl was higher than 5.0g· L- 1. It inhibited the net photosynthetic rate, chlorophyll content, chlorophyll fluorescence value and antioxidant enzymes activities of Brassica napus. Moreover, it led to the increase of lipid peroxidation, accumulation of reactive oxygen species and damaged the ultrastructure of chloroplasts and mitochondria in mesophyll cells. In addition, the ability of resistance to the stress of benazotin-ethyl in cultivar Zheda 619 is higher than the cuhivar Zheda 622, which is more sensitive. Therefore, this study defined the effect of benazolinethyl on Brassica napus and the findings have great significance to extend the field application of this herbicide.

关 键 词：油菜 草除灵 净光合速率 抗氧化酶系统 超微结构 

分 类 号：S565.4[农业科学—作物学]