肠致病性大肠埃希菌分离株血清型多样性分析  

Analysis of serological diversity of enteropathogenic Escherichia coli

作　　者：巴鹏斌 赵爱兰[1] 白向宁[1] 艾永循[2] 熊衍文[1] 许彦梅[1] 

机构地区：[1]江汉大学医学院,湖北武汉430056 [2]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《中国卫生检验杂志》2017年第2期153-156,159,共5页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金(81371762)

摘　　要：目的了解不同来源肠致病性大肠埃希菌(EPEC)分离株的血清型。方法采用特异性PCR方法检测大肠埃希菌不同O抗原编码基因,并以全套O抗血清复核确定O血清群;以PCR扩增和测序H抗原编码基因,经序列比对后确定H型。结果 331株EPEC分离株中,共检测到81种O血清型,其中优势O血清群为O51,占6.0%(20/331),O145占4.5%(15/331);共检测到25种H型,其中优势H型为H11(8.2%)、H2(7.9%)和H19(7.9%)。最常见的血清型为O51∶H7和O26∶H11。来自腹泻患者、健康从业人员、动物和生肉类的EPEC分离株中,分别检测到63种、22种、25种和17种O血清群。腹泻患者、动物和生肉类中的优势血清群/型分别为O51(8.7%)、O145(15.7%)和O76∶H7(21.2%),而健康人员的分离株无明显优势血清群。结论我国EPEC菌株的血清型呈现极大的多样性,无明显的优势血清群(型),传统上依靠血清群(型)对EPEC进行判断的方法存在局限性。Objective To identify the serotypes of enteropathogenic Escherichia coli（ EPEC） strains isolated from different sources in China. Methods Methods Different O antigen encoding genes of E. coli were detected by specific PCR,and O serogroups were confirmed with a complete set of O antiserum. H antigen encoding genes were amplified and sequenced by PCR,and then confirmed by comparing sequences. Results In this study,81 different O serogroups and 25 H types were detected in the331 EPEC strains. The prominent O serogroups were O51（ 20 /331,6. 0%） and O145（ 15 /331,4. 5%）. The prominent H types were H11（ 8. 2%）,H2（ 7. 9%） and H19（ 7. 9%）. The most common serotypes were O51∶H7 and O26∶H11. The strains from diarrheal patients,healthy carriers,animals and raw meats presented 63,22,25 and 17 O serogroups,respectively. The most common O serogroups among strains from diarrheal patients,animals and raw meats were O51（ 8. 7%）,O145（ 15. 7%） and O76∶H7（ 21. 2%）,respectively. But no significant common O serogroup was found in healthy carrier isolates. Conclusion These findings suggest that in China,the serotypes of EPEC strains are highly heterogeneous with no significant prominent O serotypes / serogroup being detected. There is great limitation for the traditional method defining EPEC by serology.

关 键 词：肠致病性大肠埃希菌 eae基因 血清群 血清型 

分 类 号：R446.5[医药卫生—诊断学]