Transfusion of Plasma Collected at Late Phase after Preconditioning Reduces Myocardial Infarct Size Induced by Ischemia-reperfusion in Rats In vivo  

作　　者：Yang Zhao Zhi-Nan Zheng Chi-Wai Cheung Zhi-Yi Zuo San-Qing Jin 

机构地区：[1]Department of Anesthesia, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China [2]Department of Anesthesia, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 999077, China [3]Department of Anesthesia, University of Virginia Health System, Charlottesville, Virginia 22902, USA

出　　处：《Chinese Medical Journal》2017年第3期303-308,共6页中华医学杂志（英文版）

摘　　要：Background： Plasma transfusion is a common clinical practice. Remote ischemic preconditioning （RIPC） protects organs against ischemia-reperfusion （IR） injury. Whether preconditioned plasma （PP）, collected at late phase after RIPC, could protect organs against IR injury in vivo is unknown. This study explored whether transfusion of PP could reduce myocardial inihrct size （IS） after I R in rat in vivo. Methods： Eighty Lewis rats were randomized to eight groups （n= 10 for each group）. Two groups of plasma donor rats donated plasma at 48 h after transient limb ischemia （PP） or control protocol （nonpreconditioned plasma [NPP]）. Six groups of recipient rats received normal saline （NS; NS-IR 1, and NS-1R 24 groups）, NPP （NPP-IR I and NPP-1R 24 groups）, or PP （PP-IR 1 and PP-IR 24 groups） at one or 24 h before myocardial IR. Myocardial IR consisted of 30-min left anterior descending （LAD） coronary artery occlusion and 180-rain reperIhsion. The area at risk （AAR） and infarct area were determined by double-staining with Evans blue and triphenyltetrazolium chloride. IS was calculated by inihrct area divided by AAR. This was a 3 × 2 factorial design study, and factorial analysis was used to evaluate the data. If an interaction between the fluid and transfusion time existed, one-way analysis of variance with Bonferroni correction lbr multiple comparisons was used to analyze the single effects of fluid type when the transfusion time was fixed. Results： IS in the NPP-IR I and PP-IR1 groups was smaller than in the NS-IR I group （F - 6.838, P = 0.005; NPP-IR I： 57 ± 8% vs. NS-IRI： 68± 6%, t = 2.843, P - 0.020; PP-IR I： 56 ~ 8% vs. NS-IR 1：68 ~ 6%, t - 3.102, P - 0.009）, but no significant difference was detected between the NPP-IR 1 and PP-IR 1 groups （57 ± 8% vs. 56 ± 8%, t 0.069, P = 1.000）. IS in the N PP-IR 24 and PP-IR 24 groups was smaller than in the NS-IR 24 group （F - 24.796, P 〈 0.001： NPP-IR 24： 56% ± 7% vs. NS-IR 24：68 ± 7%, t = 3.1Background： Plasma transfusion is a common clinical practice. Remote ischemic preconditioning （RIPC） protects organs against ischemia-reperfusion （IR） injury. Whether preconditioned plasma （PP）, collected at late phase after RIPC, could protect organs against IR injury in vivo is unknown. This study explored whether transfusion of PP could reduce myocardial inihrct size （IS） after I R in rat in vivo. Methods： Eighty Lewis rats were randomized to eight groups （n= 10 for each group）. Two groups of plasma donor rats donated plasma at 48 h after transient limb ischemia （PP） or control protocol （nonpreconditioned plasma [NPP]）. Six groups of recipient rats received normal saline （NS; NS-IR 1, and NS-1R 24 groups）, NPP （NPP-IR I and NPP-1R 24 groups）, or PP （PP-IR 1 and PP-IR 24 groups） at one or 24 h before myocardial IR. Myocardial IR consisted of 30-min left anterior descending （LAD） coronary artery occlusion and 180-rain reperIhsion. The area at risk （AAR） and infarct area were determined by double-staining with Evans blue and triphenyltetrazolium chloride. IS was calculated by inihrct area divided by AAR. This was a 3 × 2 factorial design study, and factorial analysis was used to evaluate the data. If an interaction between the fluid and transfusion time existed, one-way analysis of variance with Bonferroni correction lbr multiple comparisons was used to analyze the single effects of fluid type when the transfusion time was fixed. Results： IS in the NPP-IR I and PP-IR1 groups was smaller than in the NS-IR I group （F - 6.838, P = 0.005; NPP-IR I： 57 ± 8% vs. NS-IRI： 68± 6%, t = 2.843, P - 0.020; PP-IR I： 56 ~ 8% vs. NS-IR 1：68 ~ 6%, t - 3.102, P - 0.009）, but no significant difference was detected between the NPP-IR 1 and PP-IR 1 groups （57 ± 8% vs. 56 ± 8%, t 0.069, P = 1.000）. IS in the N PP-IR 24 and PP-IR 24 groups was smaller than in the NS-IR 24 group （F - 24.796, P 〈 0.001： NPP-IR 24： 56% ± 7% vs. NS-IR 24：68 ± 7%, t = 3.1

关 键 词：Ischemia Ischemic Preconditionina Myocardial Infarction  Myocardial Reperfusion Injury PLASMA 

分 类 号：Q255[生物学—细胞生物学] Q463