鸭瘟病毒的分离鉴定及其UL2、TK基因序列分析  

Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL2 and TK Genes

作　　者：刘荣昌[1] 黄瑜[1] 卢荣辉[2] 傅秋玲[1] 江斌[1] 万春和[1] 傅光华[1] 程龙飞[1] 施少华[1] 陈红梅[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心/福建省禽病防治重点实验室,福建福州350013 [2]福建农林大学动物科学学院,福建福州350002

出　　处：《福建农业学报》2016年第12期1257-1261,共5页Fujian Journal of Agricultural Sciences

基　　金：国家水禽产业技术体系项目(CARS-43);福建省畜禽疫病防控技术重大研发平台(2014N2003);福建省科技计划项目--省属公益类科研院所基本科研专项(2017R1102);福建省农业科学院科研项目(2015QC-2);"国家百千万工程人才"科研补助项目(NCNCMTPC-2009)

摘　　要：2015年,福建省福州地区2个鸭场(M18肉鸭场及蛋鸭场)发生大量鸭死亡,初诊疑似鸭瘟。为明确其病原,分别采集病死鸭肝脏、食道样品进行鸭瘟病毒特异性PCR检测和病毒分离,对获得的2株病毒分离株分别进行鸭已知病原的检测,确定均为鸭瘟病毒。对新分离鉴定的2株鸭瘟病毒株的UL2基因进行序列测定及分析发现,均具有强毒株的分子特征;经TK基因序列测定及分析表明,2株新分离的鸭瘟病毒株与强毒代表株、弱毒疫苗株的TK基因核苷酸同源性均高达98.7%。In March 2015,massive deaths happened to the M18 Duck Farm and an egg-laying duck farm in Fuzhou.It was suspected of an outbreak of duck plaque.Samples of liver and esophagus from the dead birds were collected for pathogenic identification using PCR and virus isolation.Two strains with virulent characteristics were thus identified.Subsequently,a sequence analysis on their UL2 genes further confirmed the classification.In addition,the TK gene sequences of the two isolated virus strains showed a nucleotide homology of 98.7% with those of the common virulent strain as well as the vaccine strain.

关 键 词：鸭瘟病毒 分离鉴定 UL2基因 TK基因 序列分析 

分 类 号：S858.32[农业科学—临床兽医学]