清热活血方药对CIA大鼠Wnt信号通路成骨细胞相关因子的影响  

Effect of Qingre Huoxue Recipe on the Wnt Signal Pathway Related Factors in CIA Rats

作　　者：李波[1] 姜泉[1] 巩勋[1] 吴振宇[1] 唐晓颇[1] 杨卉[1] 焦娟[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国中医基础医学杂志》2017年第1期75-77,99,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81273719)-从DKK/Wnt信号通路探讨清热活血方药治疗类风湿性关节炎的护骨机制

摘　　要：目的:分析清热活血方(QRHX)对类风湿关节炎大鼠Wnt信号通路相关因子表达的影响,探讨清热活血方药对CIA大鼠骨破坏的保护机制。方法:SD大鼠40只按随机数字表法分为正常组、模型组、清热活血方药组、依那西普组。通过滑膜病理切片观察各组大鼠关节滑膜病理改变,采用RT-PCR方法对各组大鼠滑膜DKK-1、β-catenin基因mRNA表达水平进行对比分析,通过ELISA检测各组大鼠血清DKK-1、β-catenin、TCF、LRP5的蛋白含量。结果:滑膜病理显示清热活血方药组大鼠滑膜中的炎症水平较模型组明显减轻,清热活血方药组大鼠滑膜中DKK-1mRNA表达水平和血清中DKK-1含量较模型组明显减少,β-catenin mRNA表达水平及血清β-catenin、TCF、LRP5含量与模型组比较显著升高。结论:清热活血方药能降低滑膜炎症水平,抑制Wnt信号通路中DKK-1细胞因子的表达,促进β-catenin、TCF、LRP5的表达,可能通过作用于Wnt/β-catenin通路在CIA大鼠骨破坏过程中发挥骨保护作用。Objective： To study the effect of QingreHuoxue Recipe （QRHX） on the expression of Wnt signaling pathway related factors in collagen-induced arthritis （CIA） rats, thus exploring the bone protection mechanism. Methods： 40 SD rats were randomly divided into the normal group, the model group, the QRHX group and the etanercept group. The changes of synovium pathomorphology were observed using synovial pathological. The DKK-1 and beta -catenin gene expression level of mRNA in rats synovial were analyzed by using RT-PCR method, and the protein content of DKK-1, beta -catenin, TCF, LRP5 beta in serum Were detected by ELISA. Results： Compared with the model group, the synovium inflammation level significantly decreased in the QRHX group, and the DKK-1 mRNA expression in synovial and DKK-I levels in serum decreased in QRHX group. The beta -catenin gene expression level and serum beta -catenin TCF, LRP5 content increased. Conclusion： QRHX Recipe could reduce the inflammation level, and play the bone protection effects by inhibiting expression of DKK-1 cytokines in the Wnt signaling pathway, increasing the expression of β-catenin, TCF, LRP5, beta cell factors, and promoting the formation of osteoblast.

关 键 词：类风湿关节炎 WNT信号通路 清热活血方 骨保护作用 

分 类 号：R285.5[医药卫生—中药学]