针刺手厥阴经穴对心肌缺血再灌注损伤大鼠CRTmRNA表达率的影响  

Effects of Electroacupuncture at the Points of the Pericardium Meridian in rate of Calreticulin Gene Expression in Myocardial Ischemia and Reperfusion Injury

作　　者：田岳凤[1] 袁叶[2] 王军[1] 李雷勇[3] 

机构地区：[1]山西中医学院针灸推拿学院,太原030024 [2]山西中医学院附属医院,太原030024 [3]山西医科大学第二医院,太原030001

出　　处：《中国中医基础医学杂志》2017年第1期105-107,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(30873240)-针刺对缺血再灌注损伤心肌细胞CaMKⅡ信号传递作用及Akt心肌保护作用的研究;山西省自然科学基金资助项目(2010011056-1)-针刺激活Akt信号通路对再灌注损伤心肌细胞凋亡干预机制的研究

摘　　要：目的:观察电针手厥阴经"内关""郄门"穴对缺血再灌注损伤过程大鼠心肌组织CRTmRNA表达率的影响,探讨经穴与脏腑间的特异性联系。方法:50只Wistar大鼠根据随机数字表法分为假手术组、缺血-再灌注模型组、针刺"内关"治疗组、针刺"郄门"治疗组以及针刺"合谷"对照组5组各10只。除假手术组外,其他4组均制备缺血-再灌注动物模型,假手术组穿线但不结扎冠状动脉。"内关"、"郄门"、"合谷"3组穴位针刺20 min后,结扎冠状动脉左前降支40 min,再次针刺上述穴位20 min松扎,恢复冠脉灌流60 min后摘取心脏取心尖部组织,采用RT-PCR方法测定CRTmRNA表达率,实验过程中心电图(ECG)全程监测。结果:假手术组CRTmRNA的表达很低,模型组与"内关"组、"郄门"组比较,CRTmRNA表达率下降非常明显;模型组与"合谷"组比较,CRTmRNA表达率比较差异无统计学意义;"内关"组与"郄门"组比较差异无统计学意义。结论:针刺手厥阴经穴可有效促进CRTmRNA的表达,提高CRTmRNA的表达率,减轻胞浆钙超载对心肌细胞的损伤。Objective：To investigate the effects of electroacupuncture （EA） of the points at the Pericardium Meridian of Hand-Jueyin on the changes of gene expression rates of CRTmRNA in myocardial ischemia and reperfusion injury rats so as to explore the specific relation between Meridian and Zangfu. Methods：Fifty Wistar rats anesthetized by 10% urethane were evenly randomized into sham, model, EA-＂ Neiguan＂ （ PC6 ）, EA-＂ Ximen＂ （ PC5 ）, EA-＂ Hegu＂ （ LI4 ） groups. Under artificial respiration, MI/R model was established by ligation of the left descending anterior branch （ DAB ） of the coronary artery for 40 rain, followed by reperfusion for 60 rain. EA was applied to the above mentioned acupoints for 20 rain , two times altogether. Then, the myocardial sample （below the ligationsite of DAB） of the left cardiac ventricle was taken for observing the changes gene expression of CRTmRNA. Results： The expression of sham group is less. Model group compared with ＂Neiguan＂ group, ＂Ximen＂ group, CRTmRNA expression is reduced very significant ; model group compared with the ＂ Hegu＂ group, CRTmRNA expression was no significant difference; between ＂Neiguan＂ and ＂Ximen＂ group there was not significantly different. Conclusions：The points of the Pericardium Meridian can obviously improve the gene expression rate of CRTmRNA, reduce injury caused by the calcium overload of myocardial cell.

关 键 词：针刺 心肌缺血再灌注损伤 钙网蛋白 反转录聚合酶链式反应 

分 类 号：R246.9[医药卫生—针灸推拿学]