5-氟尿嘧啶分子印迹传感器的制备与应用研究  

Preparation and Aplication of 5-fluorouracil molecularly imprinted Polymer sensor

作　　者：吴显兰 李柏松[2] 张继旺[1] 李英杰[1] 李远[1] 袁永强[1] 

机构地区：[1]重庆医科大学附属永川医院中心实验室,重庆402160 [2]重庆市疾病预防控制中心,400010

出　　处：《检验医学与临床》2017年第4期466-469,共4页Laboratory Medicine and Clinic

基　　金：重庆市自然科学基金项目(cstc2012jjA10046);重庆市永川区创新能力建设平台项目(Ycstc2014bf5001);重庆医科大学附属永川医院院级重点研究资助项目(YJZD201302)

摘　　要：目的研究一种用于检测抗肿瘤药物5-氟尿嘧啶(5-FU)血药浓度的方法。方法以5-FU为模板分子,吡咯为单体,采用循环伏安法于玻碳电极上构建一种选择性检测5-FU的分子印迹传感器。对分子印迹传感器制备过程中的条件进行优化;以铁氰化钾溶液为探针溶液,使用差分脉冲伏安法(DPV)对分子印迹传感器的性能进行研究。结果在优化后的实验条件下,5-FU浓度的负对数在(1×10^(-8))^(1×10^(-3))mol/L范围内与传感器差分脉冲伏安的峰电流差值呈线性关系,5-FU的检出限为0.646×10^(-8) mol/L,在血浆实际样品中测得的5-FU回收率为90%~97%,制得的传感器对5-FU具有良好的选择性、稳定性及重现性。结论该实验构建的传感器具有较低的检测限,操作简便,成本低,选择性好,将来有望用于抗肿瘤药物5-FU血药浓度的监测。Objective To develop a method for detecting the concentration of 5-fluorouracil in plasma.Methods A molecularly imprinted electrochemical sensor of 5-fluorouracil was prepared on the surface of glassy carbon electrode by cyclic voltammetry using 5-fluorouracil as template molecule and pyrrole as functional monomer.The preparation condition of sensor was optimized and the performance of the molecularly imprinted electrochemical sensor was measured by differential pulse voltammetry in 1 mmol/LK3[Fe（CN）6]-0.1mol/L KCl solution.Results The negative logarithm of 5-fluorouracil concentration was linear to the DPV peak current in the rang from 1×10^-8 to 1×10^-3 mol/L with a detection limits of 0.646×10^-8 mol/L,under the optimized preparation parameters.The recovery rate was 90%-97% in the plasma contain 5-fluorouracil.Molecularly imprinted electrochemical sensor of 5-fluorouracil has good selectivity,stability and reproducibility.Conclusion The sensor has the advantage of low detection limits,handiness,low cost,good selectivity,the molecularly imprinted electrochemical sensor of 5-fluorouracil can use to detect the clinical plasma concentration of 5-fluorouracil in future.

关 键 词：5-氟尿嘧啶 血药浓度 吡咯 电化学传感器 分子印迹 

分 类 号：R979.1[医药卫生—药品]