2种方法检测孕妇免疫球蛋白G抗-A(B)效价的对比研究  

Comparison of 2 methods for detection of anti-A(B) titer of IgG in pregnant women

作　　者：谭兵[1] 李青[1] 杨婷[2] 刘慧[1] 余泽波[1] 

机构地区：[1]重庆医科大学附属第一医院输血科,重庆400016 [2]重庆医科大学检验医学院,重庆400016

出　　处：《检验医学与临床》2017年第4期492-494,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨微柱凝胶法和抗人球蛋白试管法检测O型孕妇血清免疫球蛋白G(IgG)抗-A(B)效价的差异及相关性。方法采用微柱凝胶法和抗人球蛋白试管法同时检测133例O型孕妇血清IgG抗-A(B)水平,并比较2种方法的检测结果。结果 2种检测方法结果呈高度相关。若以凝集强度1+为判定终点,微柱凝胶法比抗人球蛋白试管法检测结果高出2个稀释倍数;微柱凝胶法以256作为临床参考值,试管法以64作为临床参考值,2种方法阳性检出率差异无统计学意义(P>0.05)。若微柱凝胶法以凝集强度3+为判定终点,试管法以1+为判定终点,2种方法检测结果差异无统计学意义(P>0.05)。结论 2种检测方法的一致性较好,微柱凝胶法的灵敏度高于抗人球蛋白试管法。若以凝集强度1+为判定终点,推荐微柱凝胶法正常参考值为小于256;若以凝集强度3+为判定终点正常参考值为小于64。Objective To compare a microcolumn gel test（MG）and the conventional tube test（CTT）for IgG anti-A（B）titer in serum of pregnant woman with blood type O,and to investigate the reference value of IgG anti-A（B）titer in serum of pregnant woman with blood type O by MG.Methods The titers of antibody in ABO blood in 133 cases of group O pregnant woman blood specimens were measured simultaneously used MG and CTT,and then made an comparative analysis on the test results.ResultsThe anti-A（B）titer results determined by MG and CTT were positively correlated.On average,two titers higher was found with MG that gives a 1＋reaction is referred to as the titer than CTT.No statistically significant difference in the positive rate was found between MG with titers of 256 as the critical value and CTT with titers of 64 as the critical value（P〉0.05）.No statistically significant difference result was found between MG that gives a 3＋ agglutination is referred to as judgment criteria and CTT that gives a1＋ agglutination is referred to as judgment criteria（P〉0.05）.Conclusion The two detection methods were in good consistency.The MG was more sensitive than CTT.In addition,the normal reference value of MG suggested by this study should be less than256.Or the normal reference value was still less than 64,if 3＋ agglutination is referred to as judgment criteria for MG.

关 键 词：微柱凝胶法 抗人球蛋白试管法 免疫球蛋白G抗体效价 新生儿溶血病 

分 类 号：R722.18[医药卫生—儿科]