大黄单体对脓毒症大鼠肠黏膜上皮细胞凋亡和增殖的影响  

Effects of rhubarb monomers on apoptosis and proliferation of intestinal epithelial cells of rats with sepsis

作　　者：王虑[1] 何超[1] 瞿金龙[1] 陈国林[2] 陈德昌[1] 

机构地区：[1]第二军医大学附属长征医院急救科,上海200003 [2]苏州大学附属第一医院急诊科

出　　处：《中华急诊医学杂志》2017年第2期155-160,共6页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金（81173402）.

摘　　要：目的观察脓毒症大鼠肠黏膜上皮细胞凋亡和增殖，探讨大黄单体保护肠黏膜屏障的作用机制。方法选用健康雄性大鼠80只，体质量230—250g，随机（随机数字法）分为单纯对照组（A组），脓毒症组（B组），地塞米松治疗组（c组）和各大黄单体治疗组，其中包括大黄酸治疗组（D组），大黄素治疗组（E组），3，8-二羟-1-甲基蒽醌-2-羧基酸治疗组（F组），1-O-咖啡酰基-2-（4-羟基-O-肉桂酰）-3-D-葡萄糖治疗组（G组），胡萝卜苷亚油酸酯治疗组（H组）。模型制备后24h后处死大鼠，取回肠末端组织，在光镜下观察小肠黏膜形态。制备石蜡切片，进行增殖细胞核抗原（proliferating cell nuclear antigen, PCNA）测定及行末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法TUNEL）检测凋亡细胞。应用单因素方差分析进行不同组别间的比较。结果脓毒症组大鼠正常结构消失，肠绒毛脱落，炎性细胞侵润；各治疗组黏膜萎缩较脓毒症脓毒症组明显减轻，肠绒毛损伤程度较轻，少量炎性细胞侵润；脓毒症组凋亡指数与假手术组比较有明显升高（P〈0．05），地塞米松治疗组和各大黄单体治疗组的凋亡指数较脓毒症组明显降低（P〈0．05）；脓毒症组大鼠肠黏膜上皮细胞PCNA阳性指数较假手术组显著降低（P〈0．05），而地塞米松治疗组和各大黄单体治疗组PCNA阳性指数较脓毒症组明显增高（P〈0．05），但仍低于假手术组（P〈0．05）。结论大黄单体可通过促进黏膜细胞的增殖，阻止肠黏膜细胞的凋亡，起到了保护肠道屏障功能的作用。Objective To observe apoptosis and proliferation of intestinal epithelial cells and to explore the mechanism of protective effects of rhubarb monomers on intestinal epithelial cells in septic rats. Methods Eighty male Sprague-Dawley （SD） rats （230-250 g） under anesthesia and sedation were subjected to cecal ligation and perforation （CLP）. After surgical preparation, rats were randomly （ramdom number） divided into 8 groups （n = 10 each） ： a sham group （A） [normal saline （NS） gavage] ; a sepsis group （ B ） （ NS gavage ） ; group C （ ip dexamethasone 0. 5 mg/kg immediately after CLP） （ C ） ; and rhubarb monomer 100 mg/kg in NS treated groups including： rhein group （D） , emodin group （E） , 3, 8- dihydroxy-l-methyl-anthraquinone-2-carboxylic acid group （ F ）, 1-O-caffeoyl-2- （ 4-hydroxy-O- cinnamoyl） -D-glucose group （G）, and 3, 8-dihydroxy-l-methyl-anthraquione group （H）. Animals were sacrificed 24 hrs after treatment. Intestinal histopathology, apoptosis （TUNEL） and proliferation of intestinal epithelial cells （proliferating cell nuclear antigen, PCNA） were measured. Multiple comparisons were carried out with one-way analysis of variance （ANOVA）. Results Histopathology revealed injury to the intestinal mucosal villi induced by sepsis in group B compared with group A. The injury was significantlyameliorated in groups C, D, E, F, G, and H compared with group B. The apoptosis index in group B was significantly higher than that in group A （P 〈 0. 05 ） and the apoptosis index in groups C, D, E, F, G, and H was significantly lower than that in group B （ P 〈 0. 05 ）. The PCNA positive index in group B was significantly lower than that in group A （P 〈 0. 05） , but was significantly higher in groups C, D, E, F, G, and H than that in group B （P 〈 0. 05 ）. Conclusion Rhubarb monomers can promote the proliferation of mucosal cells and prevent apoptosis of intestinal mucosal cells. In addition, rhubarb monomers may play a r

关 键 词：脓毒症 肠黏膜 凋亡 增殖 大黄单体 大鼠 

分 类 号：R459.7[医药卫生—急诊医学]