棕树心化学成分研究  

Chemical constituents from the heartwood of Trachycarpus fortunei(Hook.) H.Wendl.

作　　者：桂利利 陈立[2] 董俊兴[1,2] 

机构地区：[1]安徽医科大学,合肥230031 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2017年第1期62-64,76,共4页Military Medical Sciences

摘　　要：目的研究棕树心的化学成分。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及重结晶等方法进行分离纯化,通过波谱数据分析和理化性质鉴别进行结构鉴定。结果从棕树心95%乙醇提取物中分离鉴定了10个化合物,分别鉴定为:1,3-二羟基蒽醌(1),大叶茜草素(2),4-羟基-2,6-二甲氧基-苯甲酸(3),5-羟基-2-羟甲基-γ-吡喃酮(4),3,5-二羟基-2-甲基-γ-吡喃酮(5),3,5-二羟基-γ-吡喃酮(6),胡萝卜苷(7),对羟基苯甲酸(8),儿茶酚(9),β-谷甾醇(10)。结论 1~7为首次从该植物中分离得到,1~6为首次从棕榈属植物中分离得到。抗肿瘤体外活性筛选结果显示,化合物2对人骨肉瘤细胞株U20S、人神经母细胞瘤细胞株SY5Y均有一定的生长抑制作用,其IC_(50)值分别为18.10、26.59μg/ml;化合物5对人乳腺癌细胞株MCF-7的生长有一定的抑制作用,其IC_(50)值为37.31μg/ml。Objective To study the chemical constituents of the heartwood of Trachycarpus fortunei. Methods Compounds were isolated and purified by silica gel column,Sephadex LH-20 chromatography and recrystallization. Their structures were elucidated by physicochemical properties and spectral data. Results Ten compounds were isolated from the95% ethanol extract of the heartwood of T. fortunei and identified as 1,3-dihydroxyanthraquinone（1）,mollugin（2）,4-hydroxy-2,6-dimethoxy-benzoic acid（3）,5-hydroxy-2-hydroxymethyl-γ-pyranone（4）,3,5-dihydroxy-2-methyl-γ-pyranone（5）,3,5-dihydroxy-γ-pyranone（6）,daucosterol（7）,4-hydroxybenzoic acid（8）,pyrocatechol（9）,β-sitosterol（10）. Conclusion Compounds 1- 7 have been obtained from this plant for the first time,and 1- 6 from the genus for the first time. The screening results of antitumor activity in vitro show that the half inhibitory concentration IC50 of compound 2for human osteosarcoma cells U20 S and human neuroblastoma cells SY5Y is 18. 10 and 26. 59 μg/ml respectively.Compound 5 can inhibit the growth of human breast cancer cells MCF-7 with a half inhibitory concentration IC50 of 37. 31μg /ml.

关 键 词：棕榈 植物提取物 化学成分 结构鉴定 药物筛选试验 抗肿瘤 

分 类 号：R284[医药卫生—中药学]