苗药大果木姜子总黄酮与糖类成分含量测定  

Content Determination of Total Flavonoids and Sugar Components in Cinnamomum migao

作　　者：刘刚[1,2] 刘育辰[1,2] 康雪佳 于福来[3,4] 庞玉新[3,4] 李霞 贾金燕 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]国家苗药工程技术研究中心,贵州贵阳550025 [3]中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [4]海南省艾纳香工程技术研究中心,海南儋州571737 [5]贵州宏宇药业有限公司,贵州贵阳550018

出　　处：《安徽农业科学》2017年第3期141-144,共4页Journal of Anhui Agricultural Sciences

基　　金：贵州重大课题专项计划项目(黔科合重大专项字[2013]6011号)

摘　　要：[目的]建立测定大果木姜子中总黄酮、总糖、还原糖、多糖含量的方法。[方法]以芦丁和葡萄糖为对照品,采用紫外分光光度计测定大果木姜子中总黄酮、总糖、还原糖、多糖的含量并进行方法学考察。[结果]大果木姜子中总黄酮含量为(8.02±0.19)mg/g,总糖含量为(35.26±0.96)%,还原糖含量为(4.78±0.95)%,多糖含量为(30.53±0.69)%。[结论]该方法快速、简便、准确,可以用于大果木姜子中总黄酮、总糖、还原糖、多糖的含量测定。[ Objective ] To establish the method for determination of total flavonoids, total sugar, reducing sugar, and polysaecharide in Cinnamomum migao. [ Method ] Using rutin and glucose as control products, the contents of total flavonoids, total sugar, reducing sugar and polysaccharide in C. migao were detected by UV spectrophotometer, and experiments were conducted to investigate whether the method is feasible. [Result] The contents of total flavonoids, total sugar, reducing sugar, polysaceharide in C. migao were （8.02±0. 19 ）mg/g, （35.26 ±0. 96） % , （4.78 ± 0.95 ） % , （ 30.53 ±0. 69 ） % , respectively. [ Conclusion ] The method is rapid, simple, accurate, which is able to accomplish the content determination of total flavonoids, total sugar, reducing sugar, polysaccharide in C. migao.

关 键 词：大果木姜子 总黄酮 总糖 还原糖 多糖 紫外分光光度法 

分 类 号：S789.5[农业科学—林学]