Hi5-SF悬浮细胞系的建立及鉴定  

Establishment and Identification of Hi5-SF Suspension Cell Line

作　　者：马伟[1] 王家敏[1] 马桂兰 马祺 马花[1] 乔自林[1] 马忠仁[3] 冯玉萍[3] 

机构地区：[1]西北民族大学甘肃省动物细胞工程技术研究中心,兰州730030 [2]兰州百灵生物技术有限公司,兰州730010 [3]西北民族大学生物工程与技术国家民委重点实验室,兰州730030

出　　处：《西北农业学报》2017年第2期173-178,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：教育部"长江学者和创新团队发展计划"(IRT13091);甘肃省科技计划(144FKCA082);西北民族大学中央高校基本科研业务费专项资金(31920150029;zyp2015006)~~

摘　　要：从中国典型培养物保藏中心(CCTCC)引进High Five细胞并对其进行驯化,获得无血清悬浮驯化株(Hi5-SF)。依据《中华人民共和国药典(2010年版三部)》对其进行细胞生物学特性研究、微生物污染、病毒外源因子及细胞致瘤性检测。结果发现,Hi5-SF细胞复苏活力76.3%,生长曲线呈"S"型,最大增殖密度可达3.50×10~6 mL^(-1),群体倍增时间为26.2h;细菌、真菌及支原体检查均为阴性;血细胞吸附试验、致细胞病变试验和特异性病毒荧光抗体结合物检查结果均为阴性;致瘤性检查结果也为阴性。说明该细胞株符合兽用疫苗生产用细胞质量要求,可以在疫苗研究与生产中应用。High Five cells were introduced from CCTCC and domestecated, ,and we obtained semi-ad- herent cells to suspension growth cell line （Hi5-SF）. Subsequently, the biological characteristics, mi- crobial contamination,viral exogenous factors and tumorigenicity of Hi5-SF cell line were detected ac- cording to the Pharmacopoeia of People＇s Republic of China （3rd Edition,2010）. The results showed that the recovery vitality of Hi5-SF cells was 76.3%, the growth curve was S-shape, and maximum concentration was 3.50×10^6 cells ·mL^-1 with the population doubling time（PDT） of 26. 2 h. The cells were free of bacteria, fungi and mycoplasmas~ hemadsorption test, cytopathic test, specific virus fluorescent antibody binding test and tumorigenicity test were all negative. The resuhus indicated that the HiS-SF cell line meets the quality requirements of veterinary vaccine production.

关 键 词：HIGH five细胞 悬浮培养 质量评价 

分 类 号：S859.797[农业科学—临床兽医学]