基于真空渗入法的转基因大白菜植株的获得  

Obtaining Transgenic Plants of Chinese Cabbage Based on Vacuum Infiltration

作　　者：赵静[1] 吴俊清[1] 李庆飞[1] 张静[2] 张鲁刚[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质资源创新重点实验室,陕西杨凌712100 [2]山西农业大学园艺学院,山西太古030800

出　　处：《西北农业学报》2017年第2期248-254,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家科技支撑计划(2012BAD02B015);西北农林科技大学唐仲英育种基金;陕西省蔬菜产业体系~~

摘　　要：采用真空渗入法,将萝卜VPE1基因的干扰载体pRNAi-RsVPE1转入大白菜中,共收获2 414粒种子,在质量浓度为30mg/L的卡那霉素筛选下获得抗性株297株,其中9株经PCR检测呈阳性,转化率为0.37%。Real-time PCR检测结果表明,pRNAi-RsVPE1的转入导致转基因大白菜叶片中同源的BraVPE在RNA水平上的表达量下降,验证了转基因植株的可靠性,为进一步深入研究BraVPE基因功能奠定了基础。The vector pRNAi- RsVPE1 was transformed into Chinese cabbage by vacuum infiltration method and total 2 414 seeds were obtained. The kanamycin concentration 30 mg/L was determined to be the most suitable for selecting transgenic plants. 297 transgenic plants resistant to kanamycin were obtained,of which 9 positive transgenic plants were verified by PCR detection,and the transgenic rate was 0.37%. RT-PCR detection showed that the expression level of BraVPE in transgenic plants decreased significantly,reliability of transgenic plants was verified. Therefore ,the above results laid a foundation for further study in BraVPE gene functions.

关 键 词：真空渗入法 大白菜 BraVPE转基因 RNAI载体 

分 类 号：S634.1[农业科学—蔬菜学]