不同葡萄品种对灰霉病菌胁迫的响应  

Response of Different Grape Cultivars to Botrytis cinerea Stress

作　　者：姜彩鸽[1] 王国珍[1] 张怡[1] 邵鹏梅 

机构地区：[1]宁夏农林科学院植物保护研究所,银川750002 [2]银川市西夏区镇北堡镇人民政府农技服务中心,银川750021

出　　处：《西北农业学报》2017年第2期262-273,共12页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家科技支撑计划(2013BAD09B02);现代农业产业技术体系专项资金(CARS-30);宁夏自然科学基金(NZ1168);宁夏农林科学院科技创新先导资金(NKYQ-15-05)~~

摘　　要：为了解贺兰山东麓不同主栽葡萄品种对灰霉病的感病程度,依据不同品种抗性不同的分布特点制定相应的防治措施,提高防治效果。对各葡萄品种的内含物-花青素、酚类物、糖分、总抗坏血酸(或维生素C)及维生素E分别采用锦葵色素标准品法、Arnow试剂法、直接滴定法、荧光法(或2,6-二氯靛酚滴定法)、高效液相色谱法进行检测。结果表明:发病果实的花青素等物质的质量分数与健康果实存在明显差异,证明葡萄灰霉病的发生和花青素等物质有密切关系,质量分数较高抗病性较强,反之则弱;依不同葡萄品种被灰霉病菌侵染后各内含物的降幅进行比较判断,其抗病性强弱依次为‘赤霞珠’>‘梅鹿辄’>‘西拉’>‘雷司令’>‘霞多丽’>‘白诗楠’。由此可见,室内检测结果与田间发病表现一致,田间防治应以白色品种为重点。In order to investigate the susceptibility of various leading grape cultivars to Botrytis cine- tea in the eastern foot of Helan Mountain, and to propose the corresponding control measures based on the distribution characteristics of various cultivars with different resistances, the contents of inclusions in various grape cultivars, namely, anthocyanins, phenols, sugar, total ascorbic acid （vitamin C） and vi- tamin E were detected using malvidin standard method, Arnow method, direct titration method, fluo- rescence method （or 2,6-two chlorine indophenol titration method） and HPLC detection respectively. The results showed that the mass fractions of various substances in the pathogenetic fruit were signifi- cantly different from those of the healthy fruit. It was proved that the incidence of Botrytis cinerea was closely related to the grape inclusions, the higher the mass fraction,the stronger the disease resist- ance, and the weaker in the opposite. Judging by the decreasing amplitude of the inclusions of different grape cultivators infected by Botrytis cinerea ,the disease resistances of various grape cultivators were in following sequence： ＇ Cabernet＇ 〉 ＇ Merlot＇〉 ＇ Syrah＇ 〉＇ Riesling＇ 〉 ＇ Chardonnay＇ 〉 ＇ White Shi- nan＇. The lab detecting results were consistent with the field incidence,and the field control should be focused on white cultivars.

关 键 词：葡萄 灰霉病菌 内含物监测 抗病性 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治]