盐度及pH突变对大竹蛏稚贝抗氧化酶活力的影响  

Effects of changes in salinity and pH on antioxidant enzyme activity in juvenile razor clam Solen grandis

作　　者：高杉[1] 周遵春[1] 董颖[1] 曹琛 王摆[1] 姜北[1] 张乾 匡少华 郝咏芳 

机构地区：[1]辽宁省海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室,辽宁大连116023 [2]营口市水产科学研究所,辽宁营口115000

出　　处：《大连海洋大学学报》2017年第1期62-67,共6页Journal of Dalian Ocean University

基　　金：现代农业产业技术体系建设专项(nycytx-47);辽宁省科技计划项目(2014203006);辽宁省海洋与渔业厅项目(201301)

摘　　要：为了解盐度、pH对大竹蛏Solen grandis稚贝抗氧化酶活力的影响,在不同盐度(15、20、30、35)和pH(7.0、7.5、8.5、9.0)突变下,分别在0、4、12、24、48、72、96 h测定了壳长为(1.33±0.10)cm大竹蛏稚贝超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的变化。结果表明:盐度20组12、24h及盐度30组24 h,大竹蛏稚贝SOD活力显著高于对照组(盐度25)(P<0.05),盐度20组4、12 h和盐度30组12、24 h时,大竹蛏稚贝CAT活力显著高于对照组(P<0.05),48~96 h时,盐度20、盐度30组的两种抗氧化酶活力逐渐恢复至对照组水平;盐度15、35组48~96 h时,大竹蛏稚贝的SOD和CAT活力显著高于对照组(P<0.05),pH 7.5、pH 8.5组12~96 h时,大竹蛏稚贝的SOD和CAT活力显著高于对照组(pH 8.0)(P<0.05)(除pH 8.5组48 h外);pH 7.0、pH 9.0组12~96 h时,其SOD活力显著高于对照组(P<0.05),pH 7.0和pH 9.0组72 h和96 h时,CAT活力显著低于对照组(P<0.05)。研究表明:盐度和pH突变对大竹蛏稚贝SOD和CAT活力均有影响,大竹蛏稚贝对盐度20和盐度30突变有一定适应性;盐度15、盐度35和pH 7.5、pH 8.5突变后,大竹蛏稚贝长时间处于氧化应激状态;pH 7.0和pH 9.0突变可能会造成大竹蛏稚贝细胞氧化损伤;生产实践中,大竹蛏稚贝培育的盐度和pH范围分别控制在20~30和7.5~8.5较为合适。Superoxide dismutase （ SOD ） and catalase （ CAT ） activities of juvenile razor clam Solen grandis with shell length of（1. 33 cm ±0. 10 c m ） were monitored at a salinity of 15,2 0,30 and 35 and p H of 7. 0,7. 5,8. 5 and 9. 0 for 0,4, 12,24,48,72 and 96 h, respectively,in order to clarify the effects of salinity and pH on the antioxidant enzyme activities of juvenile razor clam. T he results showed that there were significantly higher activities of SOD in the clam exposed to a salinity of 20 for 12 and 24 h and a salinity of 30 for 24 h than those in the control group （ salinity 25） （ P 〈0. 05）. However,there were significantly higher CAT activities in the clam exposed to a salinity of 20 for 4 and 12 h and a salinity of 30 for 12 and 24 h than those in the control group （ salinity 25） （ P 〈 0.05）,finally activities of both SOD and CAT being recovered to the control level in 48 h and 96 h. T he razor clam exposed to a salinity of 15 and 35 from 48 h to 96 h had significantly higher S O D and C A T activities than those in controls （ P 〈0. 05） did. T he razor clam exposed to pH 7.5 and pH 8.5 from 12 h to 96 h had significantly high-er SOD and CAT activities than those in control group did （ pH 8. 0 ,except p H 8. 5 in 48 h ） （ P 〈0. 05）. There was significantly higher SOD activity in the clam exposed to p H 7. 0 and p H 9. 0 than those in the control group at 12-96 h （ P〈0. 05 ）,the activities of CAT being significantly lower from 72 h to 96 h （ P〈0. 05 ）. T he findings in-dicated that SOD and CAT activities in razor clam were affected by changes in salinity and p H , the razor clam being adaptable to sudden change in salinity within 20 and 30,and that the razor clam showed cellular oxidative damage induced by the pH 7. 0 and pH 9. 0 changes under the status of oxidative stress for a long period of exposure at a sa-linity of 15 and 35 and pH 7.5,and 8.5. T he adaptive salinity and pH are suggested to should be kept in 20-30 and 7. 5-8. 5 for the culture

关 键 词：大竹蛏 盐度 PH 超氧化物歧化酶 过氧化氢酶 

分 类 号：S968.3[农业科学—水产养殖]