蛋白质组末端肽富集策略研究进展  

Progress in strategies for enrichment of proteome terminal peptides

作　　者：陈玲凡 单亦初[1] 张丽华[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁大连116023 [2]中国科学院大学,北京100049

出　　处：《色谱》2017年第3期229-236,共8页Chinese Journal of Chromatography

基　　金：国家自然科学基金项目(21475127,21235005);国家重大科学仪器设备开发专项(2012YQ120044-8)~~

摘　　要：蛋白质的末端不仅是蛋白质合成的起点和终点,更参与并调节蛋白质的各项生理功能。研究蛋白质的末端不仅对于蛋白质的结构和功能注释十分必要,还为揭示蛋白酶的作用机理提供重要信息。该文着重针对近年来蛋白质组末端肽富集策略方面取得的进展进行综述,并对蛋白质末端组学技术在蛋白酶作用底物和位点分析中的应用进行了概述。此外,还对蛋白质末端组学研究的发展前景进行了展望。Termini of proteins are not only the origin and destination of protein synthesis, but also participating in and mediating a host of physiological functions of proteins. The determina-tion of proteins termini provides valuable information for protein structure and function annota-tion, and helps the profiling of proteases substrates and cleavage sites. Herein, the progress in the strategies for enrichment of protein terminus is reviewed. In the meantime, the applications of terminome analysis for the identification of protease substrate and cleavage site are briefly introduced. Finally, the future of terminome research is discussed.

关 键 词：蛋白质末端组学 末端肽富集 蛋白酶作用底物和位点 综述 

分 类 号：O658[理学—分析化学]