功能化介孔硅材料的合成及其对富含裸露组氨酸蛋白质的选择性吸附  

Synthesis of functional mesoporous silica and selective adsorption of surface-exposed histidine proteins

作　　者：王娇娇[1] 杨霞霞[1] 刘晓燕[1,2] 张海霞[1,2] 

机构地区：[1]兰州大学化学化工学院,甘肃兰州730000 [2]兰州大学中卫高新技术研究院,宁夏中卫755000

出　　处：《色谱》2017年第3期255-259,共5页Chinese Journal of Chromatography

基　　金：国家自然科学基金项目(21105039;21375052)~~

摘　　要：通过自由基聚合反应合成了具有高比表面积的介孔硅(PPOSS),并对其表面进行修饰和铜离子(Cu^(2+))的固定,最终得到功能化介孔硅材料(PPOSS-IDA-Cu^(2+)),然后利用PPOSS-IDA-Cu^(2+)中的Cu^(2+)与蛋白质裸露组氨酸的螯合作用,选择性吸附富含裸露组氨酸的蛋白质。对牛血红蛋白、牛血清白蛋白、肌红蛋白和溶菌酶的吸附结果表明,所合成的PPOSS-IDA-Cu^(2+)材料对富含组氨酸的牛血红蛋白有较好的吸附选择性和较大的吸附容量(3 150mg/g),并且有望与其他材料联合使用,以检测到更多相对丰度较低的蛋白质,丰富人类蛋白质组学信息,为疾病的临床诊断及病理研究提供帮助。The Cu2＋ immobilized mesoporous silica particles （PPOSS- IDA-Cu2＋） were fabrica-ted. The prepared materials were applied to the highly specific separation of surface-exposed histidine proteins,based on immobilized metal ion affinity chromatography. The adsorption experiments of bovine serum albumin,myoglobin,lysozyme and bovine hemoglobin （BHb） exhibited that PPOSS- IDA-Cu2＋ had excellent performance in the separation of protein BHb. The maximum binding capacity of the proposed materials for BHb was 3 150 mg/g. The results also demonstrated that PPOSS- IDA-Cu2＋ had potential application in removing highly abundant pro-teins in proteomic analysis,and can be combined with other techniques to get more information on low abundant proteins.

关 键 词：自由基聚合 介孔硅 聚多面体低聚倍半硅氧烷-亚氨基二乙酸-Cu2+ 裸露组氨酸 牛血红蛋白 

分 类 号：O658[理学—分析化学]