Integrative analysis for the role of long non-coding RNAs in radiation-induced mouse thymocytes responses  

作　　者：Hui Gao Zhuo Dong Wei Wei Lihong Shao Linlin Jin Yahui Lv Gang Zhao Shunzi Jin 

机构地区：[1]Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China [2]Department of Orthopedics, The First Hospital of Jilin University, Changchun 130021, China

出　　处：《Acta Biochimica et Biophysica Sinica》2017年第1期51-61,共11页生物化学与生物物理学报（英文版）

摘　　要：Long non-coding RNAs （IncRNAs） are a critical class of regulatory molecules involved in a variety of biological functions; however, their role in immune cells response to radiation is unknown. Therefore, in this study we used integrative analysis to determine the expression profile of IncRNAs in mouse thymocytes and the potential functions of IncRNAs in response to radiation. Microarray data profiling indicated that 53 IncRNAs （36 up-regulated and 17 down-regulated） and 74 coding genes （39 up-regulated and 35 down-regulated） were highly differentially expressed in the high dose radiation （HDR） group compared with the control group. In the low dose radiation （LDR） group, only one IncRNA was down-regulated. Moreover, as compared with the contro group, 109 IncRNA pathways in the HDR group and 14 IncRNA pathways in the LDR group were differentially expressed. Our data revealed the expression pattern of IncRNAs in mouse thymo- cytes and predicted their potential functions in response to LDR and HDR. In the HDR group, GO analysis showed that the role of IncRNAs in damage responses of thymocytes to HDR mainly involved chromatin organization and cell death. These findings might improve our understanding of the role of IncRNAs in LDR- and HDR-induced immune cells and provide a new experimental basis for further investigation.Long non-coding RNAs （IncRNAs） are a critical class of regulatory molecules involved in a variety of biological functions; however, their role in immune cells response to radiation is unknown. Therefore, in this study we used integrative analysis to determine the expression profile of IncRNAs in mouse thymocytes and the potential functions of IncRNAs in response to radiation. Microarray data profiling indicated that 53 IncRNAs （36 up-regulated and 17 down-regulated） and 74 coding genes （39 up-regulated and 35 down-regulated） were highly differentially expressed in the high dose radiation （HDR） group compared with the control group. In the low dose radiation （LDR） group, only one IncRNA was down-regulated. Moreover, as compared with the contro group, 109 IncRNA pathways in the HDR group and 14 IncRNA pathways in the LDR group were differentially expressed. Our data revealed the expression pattern of IncRNAs in mouse thymo- cytes and predicted their potential functions in response to LDR and HDR. In the HDR group, GO analysis showed that the role of IncRNAs in damage responses of thymocytes to HDR mainly involved chromatin organization and cell death. These findings might improve our understanding of the role of IncRNAs in LDR- and HDR-induced immune cells and provide a new experimental basis for further investigation.

关 键 词：long non-coding RNAs radiation effects mouse thymocytes response mechanism 

分 类 号：Q522[生物学—生物化学] Q274