Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging  

作　　者：Yuexin Zhou Ping Wang Feng Tian Ge Gao Lei Huang Wensheng Wei X Sunney Xie 

机构地区：[1]Biodynamics Optical Imaging Center (BIOPIC), School of Life Sciences, Peking University, Beijing 100871, China [2]Beijing Advanced Innovation Center for Genomics ([CG), Peking University, Beijing 100871, China [3]State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China [4]peking-Tsinghua Center for Life Sciences (CLS), Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China [5]Center for B ioinformatics, School of Life Sciences, Peking University, Beijing 100871, China [6]Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 01238, USA

出　　处：《Cell Research》2017年第2期298-301,共4页细胞研究（英文版）

基　　金：We thank Dr Yujie Sun for providing the dCas9-EGFP expressing plasmid and Dr Ping Wei from the Center of Quantitative Biol- ogy for the Tet-on 3G plasmids. We thank Shaoshuai Meng for his early involvement in this project. We appreciate the assistance of Bo He, Junya Zhao and Zujia Wang from Central South University in karyotype analysis. We are grateful to Xiaochen Li, Xuemei Hao and Chunyan Shan in Peking University imaging user facility for their help of acquiring cell division movies and N-SIM images. We thank Dr Patty Purcell for editing of the manuscript. Meanwhile we appreciate the assistant of Huan Hu for the guidance of FISH protocol. This work is supported by grants from the National Natural Science Foundation of China, NSFC 21390412 and NSFC21327808 for XSX, and NSFC31430025, NSFC31170126, and NSFC81471909 for WW, and in part by a grant from National Basic Research Program of China, 2015AA020407 for LH.

摘　　要：Dear Editor, Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is often abnormal （e.g., more than two）, and therefore probing chromosome copy numbers can aid cancer diagnosis.

关 键 词：活细胞成像 染色体 动态可视化 细胞生物学 拷贝数 癌细胞 

分 类 号：Q343.2[生物学—遗传学] TB873[一般工业技术—摄影技术]