产气肠杆菌新德里金属β-内酰胺酶质粒分子特征分析  

Characteristics of New Delhi metallo-beta-lactamase plasmids in Enterobacter aerogenes isolates

作　　者：周丽莉 沈勇 孙萍 张瑞丽 洪锡田 盛家和 ZHOU Li-li SHEN Yong SUN Ping ZHANG Rui-li HONG Xi-tian SHENG Jia-he(Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450000, Chin)

机构地区：[1]郑州大学附属肿瘤医院检验科,河南郑州450000

出　　处：《中华医院感染学杂志》2017年第4期729-731,738,共4页Chinese Journal of Nosocomiology

摘　　要：目的分析产气肠杆菌携带blaNDM-1质粒的转移性、质粒复制子分型及blaNDM-1的基因环境。方法产气肠杆菌HN-NDM0711为研究对象,接合实验鉴定质粒转移性,PCR方法对质粒复制子分型,染色体步移技术对blaNDM-1上下游测序,基因组序列比对使用BLASTN和BLASTP,注释使用Vector NTI 11.5.1。结果产气肠杆菌HN-NDM0711接合实验阳性,质粒复制子为IncA/C型;blaNDM-1位于不常见的插入序列ISAba14和IS91之间;在blaNDM-1上游出现一个Tn3转座子和I型整合子,整合子上含有一个由庞大镶嵌序列构成的罕见耐药基因盒。结论 IncA/C型质粒pHN-NDM0711携带blaNDM-1及耐药基因盒来源于不同抗菌药物选择压力环境下的基因重组。只有严格控制临床、工业和农业抗菌药物的合理使用才能从源头上减少此类细菌的产生。OBJECTIVE To understand the transitivity of blaNDM-1plasmid,plasmid replicon typing,and genetic environment in Enterobacter aerogenes isolates.METHODS The E.aerogenes isolates were recruited as the study objects,then the transitivity of the plasmids was identified via plasmid binding assay,the plasmid replicon typing was carried out by using PCR,the DNA downstream and upstream around blaNDM-1were sequenced by means of DNA walking method,and the genomic sequences were aligned by using BLASTN and BALSTP and were annotated with the use of Vector NTI 11.5.1.RESULTS The E.aerogenes isolates were positive for the plasmid binding assay,the plasmid rellicon was type IncA/C.The blaNDM-1gene localized between ISAba14 and IS91.Class 1integron,followed by Tn3 transposon,was identified in the upstream of the blaNDM-1,and the class 1integron bore an unique resistance gene cassette consisting of large mosaic sequences.CONCLUSION The pHN-NDM0711 bears blaNDM-1gene in an IncA/C plasmid,and the resistance gene cassette derives from gene recombination under the pressure of selection of different antibiotics.The reasonable use of clinical,industrial,and agricultural antibiotics may prevent such species of pathogen from emerging.

关 键 词：产气肠杆菌 NDM-1 Β-内酰胺酶 

分 类 号：R378.2[医药卫生—病原生物学]