阿霉素对肾上腺皮质癌细胞系SW-13克隆特性的影响  

Effect of adriamycin on the characteristics of colony derived from human adrenal cortical carcinoma cells SW-13

作　　者：曾文清[1] 陈小舟 黄振兴[3] 杨海燕[3] 邝晓聪[4] 罗佐杰[3] 

机构地区：[1]浙江中医药大学附属第二医院,杭州市310005 [2]广西南溪山医院,广西桂林市541002 [3]广西医科大学第一附属医院内分泌科,南宁市530021 [4]广西医科大学基础医学院病生教研室

出　　处：《实用医学杂志》2017年第3期343-346,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81060220);广西教育厅项目(编号:12MS061);浙江中医药大学校级课题(编号:2014ZY17)

摘　　要：目的:研究阿霉素在体干预对肾上腺皮质癌系SW-13克隆特性的影响。方法:通过建立阿霉素干预裸鼠连续传代SW-13细胞系皮下移植瘤模型,将空白组及实验组细胞进行单细胞克隆行成试验,观察单个细胞生长曲线,并计算克隆形成率及3种克隆(全克隆、部分克隆和旁克隆)的百分比,并进行Hoechst33342试验检测细胞的耐药性。结果:SW-13细胞系经过阿霉素干预裸鼠连续传代移植瘤模型后,克隆形成率较空白对照组增加(P<0.05);全克隆的百分比高于空白对照组(P<0.05);经Hoechst33342试验发现经阿霉素干预肾上腺皮质癌细胞荧光减弱。结论:阿霉素在体干预提高肾上腺皮质癌细胞系SW-13的克隆形成能力及全克隆形成能力,并提高其耐药性。Objective To explore the effect of adriamycin on the characteristics of colony derived from human adrenal cortical carcinoma cells （ACC） SW-13. Methods Treatment with Adriamycin （ADM） was used in BALB/c-nude mouse tumor xenograft model established using the ACC cell line SW-13. The characteristic of colony was assessed for the formation rates, the percentage of three colony types and growth curve of single cell. Hoechst33342 dyeing test was used to test drug resistance. Results The Single-cell colony formation rate of experimental group were significantly higher than control group （P 〈 0.05）, and the holoclone percentage of experimental group were significantly higher than control group （P 〈 0.05）. In the Hoechst33342 dyeing test, the fluorescence intensity of control was higher than experimental group. Conclusion The treatment of ADM in vivo is beneficial for the colony formations of ACC cell and the formations rate of holoclone, and can improve the ability of drug resistance of ACC cell SW-13.

关 键 词：肾上腺皮质肿瘤 克隆 全克隆 阿霉素 BALB/c-nu裸鼠 

分 类 号：R96[医药卫生—药理学]