高速逆流色谱分离纯化番泻叶中的苷类化合物  

Separation and purification of glycosidic compounds from Folium Sennae by high-speed counter-current chromatography

作　　者：段文娟[1] 吴秋霞[1,2] 李月[1] 杨国红[1] 时新刚[1] 孙卫红[2] 王晓[1] Duan Wen-juan WU Qiu-xia Li Yue Yang Guo-hong SHI Xin-gang Sun Wei-hong WANG Xiao(Shandong Provincial Key Laboratory for TCM Quality Control Technology, Shandong Analysis and test Center, Jinan 250014 China School of Food and Biology Engineering, Jiangsu University, Zhenjiang 212013, China)

机构地区：[1]山东省中药质量控制技术重点实验室,山东省分析测试中心,山东济南250014 [2]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《山东科学》2017年第1期15-19,共5页Shandong Science

基　　金：三院联合基金(ZR2016YL006);山东省科学院先导项目;山东省泰山学者岗位

摘　　要：采用高速逆流色谱法从中药番泻叶中快速分离得到4个苷类化合物,溶剂体系为正丁醇-水(9:10,V/V),流速2.0 m L/min,检测波长280 nm,转速860 r/min。从200 mg番泻叶正丁醇萃取物中分离得到10 mg芹菜素-6,8-二-C-葡萄糖苷、9 mg山奈酚-3-O-β-D-龙胆二糖苷、15 mg异鼠李素-3-O-β-D-龙胆二糖苷和5 mg番泻苷B,纯度分别为97.6%、98.9%、98.3%和99.3%。该方法稳定、高效,适合番泻叶中苷类化合物的分离纯化。Four glycosidic compounds were rapidly separated from the n-butyl alcohol of Folium Sennae by high-speed countercurrent chromatography with the solvent system of n-butanol-water （9：10, V/V）. The flow rate is 2.0 mL/min, the detection wavelength is 280 rim, and the rotate speed rating is 860 r/min. Separated from the n-butyl alcohol extracts （200 mg） of Folium Sennae, their structures were identified as apigenin-6, 8-di-C-glycoside （10 mg）, kaempferol-3-O-β - D gentiobioside （9 mg）, isorhamnetin-3-O-β-D gentiobioside （15 mg）, and sennoside B （5 mg） with the purities were respectively 97.6%, 98.9%, 98.3%, and 99.3%. The results show that this method is simple and efficient, which is suitable for the separation and purification of glycosidic compounds from Folium Sennae.

关 键 词：番泻叶 高速逆流色谱 苷类化合物 分离纯化 

分 类 号：R284.2[医药卫生—中药学]