响应曲面法优化超声提取漏芦花中总黄酮的工艺  

OPTIMIZATION EXTRACTION CONDITIONS FOR TOTAL FLAVONOIDS OF RHAPOTICUM UNIFLORUM BY RESPONSE SURFACE DESIGN

作　　者：刘丽霞[1] 包小妹[2] 刘乐乐[2] 罗素琴[2] 王玉华[2] 张英[2] 

机构地区：[1]内蒙古巴彦淖尔市医院,内蒙古巴彦淖尔015000 [2]内蒙古医科大学

出　　处：《内蒙古医科大学学报》2016年第5期393-397,400,共6页Journal of Inner Mongolia Medical University

基　　金：内蒙古医科大学青年创新基金(qncx2012024)

摘　　要：目的:在单因素的基础上,以乙醇溶为溶剂,采用响应曲面法优化超声提取漏芦花中总黄酮的工艺条件。方法:在乙醇浓度、提取时间、提取温度和液料比等单因素实验的基础上,以总黄酮得率为响应值,采用响应曲面法中的Box-Behnken模式对提取工艺进行研究。结果:最佳工艺参数为乙醇体积分数70%、提取温度70℃、料液比24.98、提取时间180min.在此条件下漏芦花总黄酮含量为0.1625μg/g。结论:该优化工艺稳定可行,可利用响应模型对实验结果进行预测。Objective：On the basis of single factor, with water and ethanol as solvent, the surface method is adopted to optimize the extraction process conditions of total flavonoids in the Rhaponticum uniflorum. Methods： On the basis of four single factors, such as ethanol concentration, ratio of solid to liquid,temperature and time, the extraction technology of the total flavonoids is studied by the Box- Behnken design of response surface methodology. Result：The optimized extraction conditions are as follows ： ethanol concentration is 70%, solid - liquid ratio is 24. 983, extractiontemperature is 70℃, extraction time is 180 min. The experimental result confirmed that under the optimal conditions, the yield of total flavonoids was up to O. 1625 μg/g. Conclusion ：The extraction technology was feasible and the response model was used to predict the experimental results.

关 键 词：漏芦花 总黄酮 响应曲面法 

分 类 号：R943[医药卫生—药剂学]