维生素C对衰老大鼠红细胞抗氧化能力的修复作用及其机制探讨  

Restore Effect of Vitamin C on the Antioxidant Capacity of Erythrocytes in Aged Rats

作　　者：王月明[1] 于振海[1] 陈仲全[1] 崔倩[1] 张慧[1] 杨赛赛[1] 盖欣欣 张娅[1] 崔钰莹 熊延连[1] Wang Yueming Yu Zhenhai Chen Zhongquan Cui Qian Zhang Hui Yang Saisai Gai Xinxin Zhang Ya Cui Yuying Xiong Yanlian(Department of Human Anatomy, College of Basic Medicine, Binzhou Medical University, Yantai 264000, Chin)

机构地区：[1]滨州医学院基础医学院人体解剖学教研室,烟台264000

出　　处：《中国细胞生物学学报》2017年第1期21-27,共7页Chinese Journal of Cell Biology

基　　金：滨州医学院科研启动基金(批准号:BY2014KYQD09);大学生科技创新课题(批准号:BY2014DKCX021)资助的课题~~

摘　　要：该文探讨了维生素C对衰老大鼠红细胞的抗氧化能力的修复作用及机制。将40只雄性SD大鼠按年龄分为4组:年轻对照组(Y)、年轻维生素C组(Yc)、年老对照组(A)和年老维生素C组(Ac)。对各组大鼠红细胞GSH、GSSG、GSH/GSSG和TFG含量进行检测。评估各组红细胞L-半胱氨酸转运能力及三价铁降低抗氧化能力差异。构建黄嘌呤/次黄嘌呤氧化酶体外氧化体系,观察各组大鼠红细胞在体外氧化条件下脂质过氧化、血红蛋白含量差异。研究发现,与年轻组大鼠相比,年老组大鼠红细胞中GSSG含量增加,GSH、TFG含量下降,GSH/GSSG比值显著降低。在年老组中,维生素C补充使TFG含量显著增加,L-半胱氨酸转运能力和三价铁降低抗氧化能力显著提升。体外氧化条件下,维生素C补充组大鼠红细胞所受氧化损伤程度显著减少,L-半胱氨酸转运能力增加。研究证明,衰老过程伴随着红细胞抗氧化能力的下降,腹腔注射维生素C可以通过提高红细胞L-半胱氨酸转运能力增加GSH抗氧化物含量,提高细胞抗氧化潜能,从而减少氧自由基诱导的损伤。The main purpose of the present study was to investigate the effects of vitamins C supplements on the antioxidant capacity of erythrocytes obtained from young and aged rats. Rats were assigned based on age to four groups： young（Y） group, young vitamin C supplement group（Yc）, aged（A） group and aged vitamin C supplement group（Ac）. The contents of GSH, GSSG, GSH/GSSG and TFG of red blood cells collected from different age stages were evaluated. L-cysteine transport and ferric-reducing antioxidant power（FRAP） value were studied. An in vitro oxidation system was constructed to explore the lipid peroxidation and methemoglobin levels. The results showed that compared with young group, the GSH content, TFG content and GSH/GSSG ratio decreased significantly, meanwhile, GSSG content increased significantly in aged group. In the aged group, vitamin C supplementation induced significantly increase in TFG content and significantly improved L-cysteine transport capacity and antioxidant capacity. Under oxidative conditions in vitro, the lipid peroxidation and methemoglobin levels and L-cysteine influx rate significantly decreased in vitamin C treatment group. We present evidence of an improvement in the antioxidant capacity of RBCs by enhancing the influx rate of L-cysteine through intraperitoneal injection of vitamin C in aged rats.

关 键 词：衰老 红细胞 维生素C L-半胱氨酸转运能力 

分 类 号：R965[医药卫生—药理学]