双载蛋白对果蝇S2细胞吞噬大肠杆菌的影响  

The Effect of Amphiphysin on the Phagocytosis of Escherichia coli by Drosophila S2 Cells

作　　者：孙保贞 朱斐[1] Sun Baozhen Zhu Fei(College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China)

机构地区：[1]浙江农林大学动物科技学院,杭州311300

出　　处：《中国细胞生物学学报》2017年第1期52-57,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31370050)资助的课题~~

摘　　要：该文研究了双载蛋白(amphiphysin,Amph)对果蝇S2细胞吞噬大肠杆菌(Escherichia coli,E.coli)的影响。在S2细胞吞入E.coli后,Amph m RNA的表达水平明显升高;但用Amph-si RNA处理后的S2细胞,其Amph蛋白质水平显著低于正常和吞噬E.coli的S2细胞。透射电镜观察发现,S2细胞可以吞噬热灭活的E.coli,且细胞内存在典型的吞噬小体,而用Amph-si RNA处理的S2细胞内则未发现吞噬小体,说明Amph可能对吞噬体形成起关键作用。Amph-si RNA处理的S2细胞对E.coli的吞噬率显著低于未用Amph-si RNA处理的S2细胞,说明敲低Amph会显著地抑制S2细胞对E.coli的吞噬率。上述结果表明,Amph在果蝇S2细胞吞噬E.coli的过程中起重要的调节作用。The effect of amphiphysin（Amph） on the phagocytosis of Escherichia coli（E. coli） by Drosophila S2 cells was studied in this paper. The observation under electron microscopy showed that heatinactivated E. coli was phagocytosed by S2 cells, and there were typical phagocytic bodies in the S2 cells. The phagocytic bodies were not found in the S2 cells treated with Amph-si RNA. After S2 cells phagocytosed E. coli, the m RNA level of Amph gene was significantly increased. However, the protein level of Amph in Amph-si RNA S2 cells was significantly lower than that of normal S2 cells and S2 cells which phagocytosed E. coli. These results indicated that Amph plays an important role in the process of phagocytosis of E. coli in Drosophila S2 cells.

关 键 词：双载蛋白 果蝇 S2细胞 吞噬 大肠杆菌 

分 类 号：Q78[生物学—分子生物学]