加味良附丸联合5-Fu对裸鼠胃癌移植瘤NF-ΚBp65表达的影响  

The Effects of Modified Liang-Fu Formula Combined with 5-Fu on the express level of NF-ΚBp65 in Transplanted tumor of gastric cancer in nude mice

作　　者：吴晓勇[1] 赵林涛[2] 宋延平[2] 许晶[3] 陈信义[4] 侯丽[4] 周义浪[5] 田杰[5] Wu Xiaoyong Zhao Lintao Song Yanping Xu Jing Chen Xinyi Hou Li Zhou Yilang Tian Jie(The First Affiliated Hospital of Guiyang College of Chinese Medicine, guiyang 550001, China Shaanxi province Pharmacological Institute of Chinese medicine, xi＇ an 710003, China Shaanxi University of Chinese Medicine, Xianyang 712046, China Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China The 2nd Affiliated Hospital of Guiyang College of Chinese Medicine, guiyang 550003, China)

机构地区：[1]贵阳中医学院第一附属医院,贵州贵阳550001 [2]陕西省中医药研究院药理室,陕西西安710003 [3]陕西中医药大学,陕西咸阳712046 [4]北京中医药大学东直门医院,北京100700 [5]贵阳中医学院第二附属医院,贵州贵阳550003

出　　处：《陕西中医药大学学报》2017年第1期76-80,共5页Journal of Shaanxi University of Chinese Medicine

基　　金：贵州省科技厅基金项目(黔科合J字[2011]2296号);贵州省科学技术厅;贵阳中医学院科学技术联合基金(黔科合中药字[2010]LKZ7034);2010年贵阳中医学院博士启动基金;北京中医药大学科学科研基地建设项目北京市重点实验室(中医内科学)(2011-JDJS-02)

摘　　要：目的观察加味良附丸联合5-Fu对人胃癌细胞SGC-7901裸鼠移植瘤生长抑制作用及其对肿瘤组织中核转录因子-kappaBp65(NF-ΚBp65)表达的影响。方法建立人胃癌细胞SGC-7901移植瘤动物模型,随机分为模型组、5-FU组及加味良附丸大、中、小剂量组、加味良附中剂量+5-FU组(联合给药组)。给药10 d,计算肿瘤抑制率,免疫组化检测肿瘤组织中NF-ΚBp65的表达。结果联合给药组、5-FU组、加味良附丸大、中、小剂量组抑瘤率分别为54%、45%、31%、28%、17%,联合给药组与加味良附丸大、中、小剂量比,差异显著有统计学意义(P<0.05);模型组肿瘤组织中NF-ΚBp65表达明显高于各给药组,差异显著有统计学意义(P<0.05);联合给药组肿瘤组织中NF-ΚBp65表达低于加味良附丸大、中、小剂量组,差异明显有统计学意义(P<0.05)。结论加味良附丸联合5FU组抑瘤率高于单纯加味良附丸高、中、低剂量组及5-FU组,联合给药抑制NF-ΚBp65表达方面优于加味良附丸高剂量组及5-Fu组,提示中西药联合具有协同作用。Objective To observe the effects of modified liang-fu formula （MLFF） combined with 5-Fu which focus on NF-KBp65 level in tumor tissues of SGC-7901 tumor bearing nude mice and the tumor growth inhibition. Methods Establish the tumor-bearing nude mice models with human gastric cancer cell line SGC- 7901, which were divided randomly into six groups ： blank group,5-Fu group, MLFF in high, medium, low dose groups, and combination group. The mice were treated for 10 days and then detecting NF-KBp65 level in tumor tissues of SGC-7901 tumor bearing nude mice and calculateing the tumor inhibition ratio. Results The tumor inhibition ratio of combination group was 54%, which had significant difference between blank group （ P〈0.05. The tumor inhibition ratio of combination group was higher than MLFF in high dose group remarkable. The contents of NF-KBp65 in combination group was lower than blank group, 5- Fu group and MLFF in high dose group respectively. Conclusion The tumor inhibition ratio of combination group is higher than 5-Fu group and MLFF in high dose group, meanwhile, the combination group is better than 5-Fu group and MLFF in high dose group on regulating expression of NF-KBp65 level in tumor tissues. Which means Modified Liang-Fu Formula Combined with 5-Fu has synergistic effects.

关 键 词：加味良附丸 5-FU SGC-7901细胞 NF-KAPPA BP65 

分 类 号：R961[医药卫生—药理学]