荷斯坦牛TLR6基因多态性分析  

Polymorphism of TLR6 Gene in Chinese Holstein Cattle

作　　者：周秀敏[1] 李强子[1] 毕英杰[1] 任卫合 张丽[1] Zhou Ximnin Li Qiangzi Zhang Li et al(College of Life Science and Engineering, Northwest University for Nalionalities, Lnzhou 730030,Chin)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《中国草食动物科学》2017年第1期1-3,共3页China Herbivore Science

基　　金：西北民族大学引进人才科研项目(xbmuyjrc201316);西北民族大学生命科学与工程学院众创空间扶持项目

摘　　要：为研究中国荷斯坦牛TLR6基因的多态性,确定其等位基因数以及核苷酸变异位点,探讨其遗传特性,采用PCR-SSCP技术对303头荷斯坦牛的TLR6基因进行了遗传多态性检测。结果表明:TLR6基因在扩增区域的209 bp处发生G→A的错义突变,导致TLR6蛋白中第70位天冬氨酸(Asp)变为天冬酰胺(Asn),并产生AA、BB、AB三种基因型,基因型频率分别为0.214 5、0.122 1和0.663 4,经χ~2适合性检验,荷斯坦牛在该位点偏离了Hardy-Weinberg平衡状态(P<0.05)。说明中国荷斯坦牛TLR6基因多态性丰富,该位点可以作为荷斯坦牛抗病育种的分子标记位点。In order to study the polymorphism of TLR6 gene in Holstein cattle, the blood samples of 303 Holstein cattle were detected by polymerase chain reaction- single strand conformation polymorphism（PCR- SSCP）. The results showed that there was a missense mutation of G to A in the TLR6 gene, which led to the change of aspartic acid to asparagine in the TLR6 protein,producing three genotypes（AA,BB,AB）with the frequencies of 0.214 5,0.122 1 and 0.663 4 respectively. The χ^2 test indicated that the gene at this site deviated from Hardy- Weinberg equilibrium（P〈0.05）. It was concluded that the polymorphism of TLR6 gene was rich and the locus could be used as a molecular marker for resistance breeding of Holstein cattle.

关 键 词：荷斯坦牛 TLR6基因 遗传多态性 

分 类 号：S826.2[农业科学—畜牧学]