响应面法优化蓝花荆芥中总黄酮提取工艺  

Response Surface Optimization of Flavonoids Extraction from Nepeta coerulescens

作　　者：叶菊[1,2] 孙立卿 吉守祥[1] 

机构地区：[1]青海民族大学青海省药物分析学重点实验室,青海西宁810007 [2]青海民族大学青海省青藏高原植物资源化学重点实验室,青海西宁810007 [3]青海民族大学化学化工学院,青海西宁810007

出　　处：《广西师范大学学报（自然科学版）》2017年第1期62-68,共7页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(81160554);青海省基础研究计划项目(2016-ZJ-712);青海省科技厅重点实验室发展专项(2015-Z-Y09);青海民族大学高层次人才项目(2016XJG04)

摘　　要：采用乙醇热浸提法,考察料液比、乙醇浓度、提取温度以及提取时间对藏药蓝花荆芥全草中总黄酮提取率的影响,并结合响应面设计方法,对藏药蓝花荆芥全草中总黄酮提取工艺进行优化。由实验得到蓝花荆芥总黄酮最佳提取工艺为:乙醇体积分数35%,提取温度65℃,提取时间70min,该条件下总黄酮提取率为0.351%。Nepeta coerulescens flavonoids were extracted with ethanol. The effects of time, temperature, the ethanol solution concentration on the content of Nepeta coerulescens were investigated in singlefactor test. The preferred extraction conditions were determined by RSM. The optimization extraction conditions by RSM analysis were obtained at ethanol concentration of 35M, temperature of 65 ℃ and time of 70 min. Under the optimization extraction conditions, the extraction ratio of flavonoids can reached 0.351%.

关 键 词：蓝花荆芥 黄酮 响应面法 优化 

分 类 号：Q946.8[生物学—植物学]