中药棕榈子质量控制方法研究  

Study on Quality Standard of Trachycarpus Fruit

作　　者：杨彦坤 王珂[1] 姚静[2] 杨柳[2] 白关亚 倪艳[2] 郝旭亮[2] 

机构地区：[1]山西中医学院中药学院,山西太原030619 [2]山西省中医药研究院中药方剂研究所,山西太原030045

出　　处：《中国医院用药评价与分析》2017年第1期18-21,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：山西省科技攻关项目(山西省中药现代化关键技术研究振东专项)(No.2014ZD0203)

摘　　要：目的:研究建立棕榈子药材的质量控制方法,提高其质量可控性。方法:采用对照药材为对照,进行棕榈子药材的薄层色谱鉴别;采用高效液相色谱法测定棕榈子药材中原儿茶酸含量,色谱柱为Hypersil Sino Chrom ODS-BP C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(V∶V=15∶85),流速0.8 ml/min,检测波长260 nm,柱温25℃,进样量10μl。结果:薄层色谱图中,斑点清晰,分离度好。含量测定中,原儿茶酸进样量在0.025 8~0.309 6μg范围内与其峰面积呈现良好的线性关系(r=0.999 7);精密度、稳定性、重复性试验的RSD分别为0.9%、1.6%、2.3%,均符合要求;平均加样回收率为97.4%,RSD为2.8%(n=9)。结论:本研究所建立的薄层色谱鉴别方法简便可行,含量测定方法快捷、准确、专属性强,可用于棕榈子药材的质量控制。OBJECTIVE： To study and establish the quality standard method of Trachycarpus Fruit,and improve its quality control. METHODS： The control medicinal materials were adopted for TLC identification of Trachycarpus Fruit and HPLC was conducted to determine the contents of protocatechuic acid of Trachycarpus Fruit. The column was Hypersil Sino Chrom ODS-BP C_（18）（ 250 mm × 4. 6 mm,5 μm）,with mobile phase of acetonitrile-0. 1% phosphoric acid water（ V ∶ V = 15 ∶ 85）,flow rate of 0. 8 ml / min,detection wavelength of 260 nm,column temperature of 25 ℃ and injection volume of 10 μl. RESULTS： TLC showed clear spots and good separation. The linear range of protocatechuic acid was 0. 025 8 μg-0. 309 6 μg（ r = 0. 999 7）. The RSD values of precision,stability,reproducibility were 0. 9%,1. 6% and 2. 3% conformed to the requirements. The average recovery rate was 97. 4% with RSD of 2. 8%（ n = 9）.CONCLUSIONS： The TLC identification method is simple and feasible,the method of content determination is rapid,accurate,specific and can be used in the quality control of Tachycarpus Fruit.

关 键 词：棕榈子 质量标准 薄层色谱 原儿茶酸 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]