LC/MS/MS测定婴幼儿配方乳粉中3种苯扎氯铵同系物  

Simultaneous Determination of Three Kinds of Benzalkonium Chloride Homologs in Infant Formula Milk Powder by LC-MS/MS

作　　者：孙龙[1] 邵明媛[1] 王浩[1] SUN Long SHAO Ming-yuan WANG Hao(China National Food Quality and Safety Supervision and Inspection Center, Beijing 100094, China)

机构地区：[1]国家食品质量安全监督检验中心,北京100094

出　　处：《食品研究与开发》2017年第1期165-169,共5页Food Research and Development

摘　　要：建立液相色谱-串联质谱法测定婴幼儿配方乳粉中3种苯扎氯胺同系物残留的方法。样品经2%乙酸水溶液和乙腈超声辅助提取。采用多反应监测模式对目标化合物进行定性和定量测定。在最优条件下,目标化合物在10μg/kg^500μg/kg范围内有较好的线性关系,检出限和定量限分别为3μg/kg和10μg/kg。方法加标回收率为85.1%~102.1%,相对标准偏差(RSD)为3.05%~8.35%。采用所建立的方法对中国市面上的婴幼儿配方乳粉进行检测,10个检测样品中均有苯扎氯胺存在,浓度范围为73μg/kg^501μg/kg。A liquid chromatography-tandem mass spectrometry（LC-MS/MS）method was established for the simultaneous determination of residues of 3 benzalkonium chloride homologs in infant formula milk powder.Samples were extracted with 2 % acetic acid aqueous solution and acetonitrile by ultrasonic. Identification and quantification of the compounds was by liquid chromatography-tandem mass spectrometry in multiple reaction monitoring（MRM）mode. Under the optimal experimental conditions,good linearity was observed in the range of 10 μg/kg-500 μg/kg. The limits of detection and quantification were 3 μg/kg and 10 μg/kg,respectively. The average spiked recoveries of the method were in the range of 85.1 %-102.1 % with the relative standard deviations（RSD）of 3.05 %-8.35 %. After validation,the method was applied to the analysis of benzalkonium chloride residues in infant formula milk powder in China. A screening of 10 samples revealed the presence of benzalkonium chloride with the concentration were in the range of 73 μg/kg-501 μg/kg.

关 键 词：液相色谱-串联质谱 苯扎氯胺 婴幼儿配方乳粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]