基于ApoE^(-/-)小鼠TLR4、MCP-1、ICAM-1表达探讨首参颗粒干预动脉粥样硬化的效应机制  

Int ervention Effect Mechanism of Shoushen Granule on Atherosclerosis Based on the Expression of TLR4,MCP-1 and ICAM-1 in ApoE-/-mice

作　　者：申定珠[1] 邢三丽[1] 陈川[1] 吴蓓玲[1] 迟惠英[1] 陈久林[1] 

机构地区：[1]上海市中医老年医学研究所,上海200031

出　　处：《中国中医急症》2017年第2期192-194,232,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81202731;81373706);上海市自然科学基金项目(16ZR1433900);上海市卫计委科研项目(201640217)

摘　　要：目的观察首参颗粒对ApoE^(-/-)小鼠Toll样受体4(TLR4)、单核细胞趋化因子-1(MCP-1)、细胞间黏附分子-1(ICAM-1)的影响。方法采用高脂饮食喂饲ApoE^(-/-)小鼠复制动脉粥样硬化(AS)模型,并给予首参颗粒、阿托伐他汀干预15周,采用Western blotting法检测小鼠主动脉TLR4蛋白表达,ELISA法检测血清MCP-1、ICAM-1表达,结合血脂及组织病理学检测。结果与正常组比较,高脂饮食喂饲ApoE^(-/-)小鼠可致明显的AS病变,TLR4、MCP-1、ICAM-1表达上调及TC、LDL-C含量升高;经首参颗粒干预,AS斑块面积显著减小,TLR4、MCP-1、ICAM-1表达下调,TC、LDL-C含量降低(P<0.05或P<0.01)。结论首参颗粒可通过下调ApoE^(-/-)小鼠主动脉TLR4蛋白表达与血清MCP-1、ICAM-1水平进而有效干预治疗AS。Objective： To investigate the effect of Shoushen Granule on the Toll like receptor 4（TLR4）,serum monocyte chemokine-1（MCP-1） and intercellular adhesion molecule-1（ICAM-1） of ApoE（-/-）mice. Methods：Animal model of atherosclerosis was made with ApoE（-/-）mice fed with high fat diet,and intervented with Shoushen Granule and atorvastatin for 15 weeks respectively. Expression of TLR4 protein in mice aorta was detected with Western blotting method,and expression of serum MCP-1and ICAM-1 was detected with ELISA method in combination with serum lipid and tissue pathology. Results： Compared with the normal group,high fat diet fed ApoE（-/-）mice could induce significant AS lesions and the expression of TLR4,MCP-1,ICAM-1,TC and LDL-C increased significantly. After intervention of Shoushen Granule,AS patch area decreased significantly,and the expression of TLR4,MCP-1,ICAM-1,LDL-C and TC decreased evidently（P 〈0.05 or P〈0.01）. Conclusion： Shoushen Granule can down regulate the expression of TLR4 protein,in mice aorta and serum MCP-1 and ICAM-1,and then can effectively intervene the treatment of AS.

关 键 词：动脉粥样硬化 TLR4 MCP-1 ICAM-1 首参颗粒 

分 类 号：R285.5[医药卫生—中药学]