土家族药三百棒提取物对大鼠肢体缺血再灌注损伤血清SOD与MDA水平的影响  

The Influence of Toddalia Asiatic Extract in Tujia Medicine on the Level of Serum SOD and MDA in Rats with Limb Ischemia Reperfusion Injury

作　　者：卢卫忠[1] 周杰[1] 姜仁建[1] 刘正敏[1] 胡志芬[1] 曹洪辉[1] 刘金坤[1] 王琴[1] 

机构地区：[1]重庆市中医院,重庆400021

出　　处：《中国中医急症》2017年第2期226-228,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：重庆市科委科研院所基本科研业务费计划项目(cstc2015jbky330025007)

摘　　要：目的观察血清超氧化物歧化酶(SOD)与丙二醛(MDA)变化,探寻三百棒提取物对大鼠肢体缺血再灌注损伤的保护作用机制。方法 66只SD大鼠随机分为对照组、缺血再灌注组和三百棒提取物干预组。对照组动物不给药物,缺血-再灌注组于缺血前12 h、缺血时和再灌注时各给予0.9%氯化钠注射液1 mL灌胃;三百棒提取物干预组于缺血前12 h、缺血时和再灌注时各给予三百棒提取物1 mL灌胃,分别于相应时间点在麻醉下抽取各组动物下腔静脉血,测量血清中SOD和MDA的含量。结果与缺血-再灌注组相比,三百棒提取物干预组各时间点大鼠血清SOD水平明显上升,而MDA水平明显下降(P<0.05)。结论三百棒提取物能增加SOD信号通路表达,减少MDA通路启动,对大鼠肢体缺血再灌注损伤具有保护作用。Objective： To investigate the protection of Toddalia asiatic extract in Tujia medicine in rats with limb ischemia reperfusion injury by observing the changes of serum SOD and MDA. Methods： 66 SD rats were randomly divided into the control group,ischemia-reperfusion group and Toddalia asiatic extract intervention group. The control group were not given drug,and ischemia-reperfusion group were gavaged with 1 mL 0.9% NS,in the first 12 h prior to ischemia and ischemia and reperfusion;the Toddalia asiatic extract intervention group in the first 12 h,ischemia and reperfusion were given 1 mL of Toddalia asiatic extracts. Inferior vena venous blood was extracted respectively in the corresponding time points in anesthesia to measure the contents of serum SOD and MDA. Results： Compared with ischemia-reperfusion group,serum SOD level in Toddalia asiatic extract intervention group increased at each time point,while the MDA level significantly decreased（P〈0.05）. Conclusions： Tujia medicine Toddalia asiatic extract can increase signal path expression of SOD,reduce pathways start of MDA with a protective effect on limb ischemia reperfusion injury in rats.

关 键 词：提取物 三百棒 缺血再灌注损伤 肢体 SOD MDA 

分 类 号：R285.5[医药卫生—中药学]