2011-2013年珠海地区诺如病毒的基因分型  

Genotypes of norovirus in Zhuhai region,2011-2013

作　　者：林毅雄 魏泉德[1] 阮锋[1] 张丽荣[1] 

机构地区：[1]广东省珠海市疾病预防控制中心,广东珠海519000

出　　处：《实用预防医学》2017年第3期364-368,共5页Practical Preventive Medicine

基　　金：珠海市医学科研基金项目(2015J022)

摘　　要：目的了解2011-2013年珠海地区诺如病毒的基因型别及分子流行病学特点。方法收集珠海地区2011-2013年急性胃肠炎暴发疫情的患者肛拭子标本619份,2011-2013年珠海市两家腹泻监测医院门诊或住院的婴幼儿粪便标本1 150份,采用ELISA法检测标本诺如病毒抗原,选取部分诺如阳性标本用RT-PCR扩增衣壳蛋白并进行测序分型。结果 28起急性胃肠炎暴发疫情其中17起由诺如病毒引起,暴发疫情标本诺如病毒抗原阳性率为19.55%(121/619);分型的标本为47例,全部为No V GⅡ,14株为GⅡ.4型,其中5株为GⅡ.4 2006b,另外9株为GⅡ.4/Sydney_2012;12株属于GⅡ.3型;3株属于GⅡ.5型;18株属于GⅡ.6型。监测标本诺如病毒抗原阳性率为33.13%(381/1 150);分型的标本为71例,全部为No V GⅡ,其中36株为GⅡ.4 2006b,另外32株为GⅡ.4/Sydney_2012,2株属于GⅡ.3型,1株是GⅡ.1型。结论珠海市诺如病毒暴发疫情以GⅡ组为主,但基因亚型存在多样性;珠海市婴幼儿监测中诺如病毒流行以GⅡ.4亚型为主。Objective To investigate the genotypes and molecular epidemiological characteristics of norovirus in Zhuhai region during 2011-2013. Methods We collected 619 anal swab specimens from patients during acute gastroenteritis outbreaks and 1,150 stool specimens from children hospitalized for diarrhea in 2 surveillance hospitals in Zhuhai region from 2011 to 2013. Norovirus antigen was detected by ELISA. Some norovirus antigen-positive stool specimens were selected to amplify the N/S gene by RT-PCR, and then PCR products were sequenced and genotyped. Results Among 28 outbreaks of acute gastroenteritis, 17 ere positive for norovirus, and 19.55%（121/619） of the anal swab specimens were positive for norovirus antigen. 47 specimens which were successfully genotyped belonged to norovirus genogroup II （Nov G II）. 14 strains belonged to Nov G II.4, including 5 Nov G II.4 2006b strains and 9 Nov G II.4/Sydney_2012 strains. 12 strains belonged to Nov G II.3, 3 strains Nov G II.5 and 18 strains Nov G II.6. 33.13%（381/1,150） of the stool specimens were positive for norovirus antigen. 71 specimens which were successfully genotyped belonged to Nov G II. 36 strains belonged to Nov G II.4 2006b, 32 strains Nov G II. 4/Sydney_2012, 2 strains Nov G II.3 and 1 strain Nov G II.1. Conclusions Nov GII is prevailing in the outbreaks of acute gastroenteritis in Zhuhai City, but the genotypes are widely distributed. Nov G II.4 is identified as the predominant genotype among children with norovirus infection in Zhuhai City.

关 键 词：诺如病毒 基因型 GⅡ型 

分 类 号：R512.5[医药卫生—内科学]