以对羟基苯甲酸和1,10菲啰啉为配体铜配合物的合成及与DNA的作用  

Synthesis and Interaction with DNA of Copper Complex Constructed from 4-hydroxybenzolate Acid and 1,10-phenanthroline

作　　者：李小芳[1] 冯小强[1] 常慧[1] 杨声[2] 

机构地区：[1]天水师范学院化学工程与技术学院,甘肃天水741001 [2]定西师范高等专科学校,甘肃定西743000

出　　处：《材料科学与工程学报》2017年第1期129-133,共5页Journal of Materials Science and Engineering

基　　金：甘肃省青年科技基金资助项目(1606RJYE247);天水师范学院"中青年"教师科研资助项目(TSA1508)

摘　　要：通过元素分析、红外光谱、紫外光谱及差热热重分析,对合成的一种新型铜配合物Cu(phen)(DPA)0.5·3H_2O(phen=1,10菲啰啉,DPA=对羟基苯甲酸)进行了结构表征,并以鲱鱼精DNA为靶点,采用光谱法、DNA粘度滴定实验和循环伏安法,研究了该配合物与DNA的键合方式。结果发现:在加入DNA后,配合物的吸收峰出现减色和红移,荧光强度增强;DNA的相对粘度随配合物的加入而增大;配合物在加入DNA后的氧化还原峰电流减小,式量电位发生正移。这些实验结果表明配合物与DNA通过嵌插方式发生作用。A novel ternary complex of Cu（phen）（DPA）0.5·3H2O（Phen=1,10-phenanthroline,DPA=4-hydroxybenzolate acid）was synthesized and characterized by elemental analysis,IR spectroscope,UV-Vis spectroscope and TG-DTA methods.Using herring sperm DNA as biomolecular target,its interaction with the complex was studied by spectroscopy,CV method and DNA viscosity titration experiment.Results showed that the UV absorption peaks of the complex were red-shifted and the intensity was weakened,and the fluorescence intensity was enhanced by the addition of DNA.In addition,the peak current decreased significantly and the potential moved positively.Viscosity titration experiment verified that the complex increased the relative viscosity of DNA.All of the above illustrated that the complex and DNA bond by the mode of intercalation.

关 键 词：对羟基苯甲酸 1 10菲啰啉 配合物 鲱鱼精DNA 

分 类 号：O629.74[理学—有机化学]