HIV感染者合并隐孢子虫的基因分型调查  

An investigation on the gene types of Cryptosporidium among HIV/AIDS patients

作　　者：苏海萍[1] 黄兴锋[1] 覃勇民[1] 覃素鲜[1] 黄中元[1] 吴宁生[1] 刘柳玲[1] 张秀红[1] 

机构地区：[1]河池市人民医院,广西547000

出　　处：《医学动物防制》2017年第2期151-153,共3页Journal of Medical Pest Control

基　　金：河池市本级财政基金计划课题(河科能1378-1)

摘　　要：目的了解河池市HIV感染者的隐孢子虫感染情况。方法收集258例HIV感染者的粪便样本,采用基于18S rRNA基因的巢式PCR方法进行检测。对巢氏PCR产物进行测序,同源性比对,明确基因型。结果河池市HIV感染者合并隐孢子虫的感染率为2.33%(6/258),伴有慢性腹泻的HIV感染者合并隐孢子虫的感染率为6.98%(6/86),虫株基因鉴定为安氏隐孢子虫和人隐孢子虫。结论河池市HIV感染者存在隐孢子虫合并感染,需明确其流行特点,有待进一步调查。Objective To understand the infection and gene types of Cryptosporidium among HIV/AIDS patients in Hechi City of Guangxi. Methods Fecal samples were collected from 258 HIV/AIDS cases. The nested - PCR based on 18S rRNA was em- ployed to detect Cryptosporidium. The nested PCR products were sequenced. Homology searches and identification for their gene types were done by DNAStar software. Results The infection rate of Cryptosporidium in HIV/AIDS patients of Hechi was 2. 33% （6/258）. The rates of Cryptosporidium in the HIV/AIDS patients with chronic diarrhea was 6. 98% （6/86）. Cryptosporidium andersoni and Cryptosporidium hominis were identified. Conclusion Cryptosporidium was found in HIV/AIDS patients in He- chi. It can be used widely deserves further surveyed.

关 键 词：HIV感染者 隐孢子虫 巢式聚合酶链反应 

分 类 号：R512.91[医药卫生—内科学] R531.5[医药卫生—临床医学]