橘核柠檬苦素类化合物积累与功能基因表达的灰色关联度分析  

Grey relational analysis of relationship between limonin compounds accumulation and functional genes expression of Citri Reticulatae Semen

作　　者：王黎[1] 罗静[1] 裴瑾[1] 王倩[1] 吴沂芸 焦梅英 

机构地区：[1]成都中医药大学中药资源系统研究与开发利用国家重点实验室培育基地,四川成都611137

出　　处：《中草药》2017年第2期345-350,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金人才项目(J131003340-13)

摘　　要：目的研究橘核传统药用品种大红袍Citrus reticulata‘Dahongpao’中柠檬苦素类化合物生物合成机制与转运规律。方法利用UPLC法测定橘种子生长发育过程中茎、叶、果皮及种子中的柠檬苦素、诺米林、黄柏酮等柠檬苦素类化合物的量,采用灰色关联度分析方法,与克隆获得的角鲨烯合成酶基因(ss)、角鲨烯环氧酶基因(se)、葡萄糖基转移酶基因(lgt)表达量进行关联度分析。结果不同器官中柠檬苦素类化合物在种子中得到最大积累;不同器官中柠檬苦素类化合物的积累与ss、se、lgt基因表达均有较大的关联,但在种子中柠檬苦素的积累与ss、se、lgt基因表达最密切,其中ss基因表达对柠檬苦素、诺米林、黄柏酮的积累贡献最大。结论本实验为柠檬苦素类化合物合成机制与转运规律提供可靠、科学的依据。Objective To study the biosynthesis mechanism and transshipment law of limonin compounds in Citri Reticulatae Semen, traditional medicinal variety Citrus reticulata ＂Dahongpao＂. Methods In the process of seed growth and development, the contents of limonin, nomilin, and obacunone in stem, vane, peel, and seeds were determined by UPLC tangerine; Using grey relational analysis method, the correlation analysis between the contents and the cloning of squalene synthase gene（ss）, squalene epoxidase gene（se）, and glucose transferase（lgt） expression was performed. Results Limonin compounds in different organs had the largest accumulation in seeds; The accumulation of limonin compounds in different organs was significantly correlated with ss, se, and lgt gene expression, but in the seeds the accumulation of limonin has the most closely correlation with ss, se, and lgt gene expression; The ss gene expression had the largest contribution on the accumulation of limonin, nomilin, and obacunone. Conclusion The study provides reliable and scientific envidendce for the synthesis mechanism and transshipment law of limonin compounds.

关 键 词：橘核 柠檬苦素类化合物 功能基因 灰色关联度分析 角鲨烯合成酶基因 角鲨烯环氧酶基因 葡萄糖基转移酶基因 

分 类 号：R282.12[医药卫生—中药学]