青叶胆化学成分的UPLC-ESI-Q-TOF-MS分析  

Analysis on chemical constituents from Swertia mileensis by UPLC-ESI-Q-TOF-MS

作　　者：王鑫[1] 陈雪晴[2] 尤蓉蓉[2] 鞠建明[2] 徐金娣[2] 范君婷[3] 周洪雷[1] 

机构地区：[1]山东中医药大学药学院,山东济南250300 [2]江苏省中医药研究院,江苏南京210028 [3]南京医科大学药学院,江苏南京211166

出　　处：《中草药》2017年第3期453-459,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年基金(31500278);江苏省高校自然科学研究面上项目(13KJB360002);山东省高校中医药抗病毒协同创新课题(XTCX2014C01-03)

摘　　要：目的运用超高效液相色谱-电喷雾四级杆飞行时间质谱(UPLC-ESI-Q-TOF-MS)技术对中药青叶胆Swertia mileensis的主要化学成分进行定性分析。方法采用ACQUITY HSS T3色谱柱(100 mm×2.1 mm,1.8μm),用0.1%甲酸乙腈-0.1%甲酸水为流动相进行梯度洗脱,体积流量为0.4 m L/min,质谱使用ESI离子源,正负离子模式下采集数据,运用Masslynx4.1软件并结合Sci Finder数据库、獐牙菜属相关文献以及对照品信息进行数据分析。结果从青叶胆中鉴定出化学成分28个,包括7个环烯醚萜类、14个酮类、3个黄酮类、2个三萜类以及2个酚类。其中断马钱子苷半缩醛内酯、8-O-β-D-吡喃木糖(1→6)-β-D-吡喃葡萄糖-1,7-二羟基-3-甲氧基酮、3-氧去甲双酮苷和sweriyunnanlactone A为首次从该植物中发现。结论 UPLC-ESI-Q-TOF-MS技术能够快速准确地鉴定青叶胆中的化学成分,为其质量控制和药效物质基础研究提供一种新的研究策略。Objective To analyze and identify the chemical constituents from Swertia mileensis by UPLC-ESI-Q-TOF-MS. MethodsThe analysis was performed on an Acquity HSS T3 reverse phase column（100 mm × 2.1 mm, 1.8 μm）. The mobile phase consisted of 0.1% formic acid acetonitrile and 0.1% formic acid, and was used for gradient elution, with the flow rate of 0.4 m L/min. Mass spectrometry was applied for the qualitative analysis under positive and negative ion modes and ESI ion source. Data was analyzed by Masslynx 4.1 software, Sci Finder database, literatures, and standards. Results Twenty-eight compounds, including 7 iridoids, 14 xanthones, 3 flavonoids, 2 triterpenes, and 2 phenols were identified from S. mileensis. Among them, vogeloside, 8-O-β-Dglueopyranosyl-（l→6）-β-D-glueopyranosyl-l,7-dihydroxy-3-ethoxyxanthone, 3-O-demethylswertipunicoside, and sweriyunnanlactone A were reported from this species for the first time. Conclusion Using UPLC-ESI-Q-TOF-MS method the main chemical constituents from S. mileensis can be rapidly and accurately identified, which provides a new strategy for its quality control as well as a reference for clarifying the material basis of its efficacy.

关 键 词：青叶胆 UPLC-ESI-Q-TOF-MS 环烯醚萜类 酮类 黄酮类 三萜类 断马钱子苷半缩醛内酯 8-O-β-D-吡喃木糖(1→6)-β-D-吡喃葡萄糖-1 7-二羟基-3-甲氧基酮 3-氧去甲双酮苷 sweriyunnanlactone A 

分 类 号：R284.1[医药卫生—中药学]