补脾强力复方对自身免疫性重症肌无力大鼠胸腺组织GR mRNA表达的影响  

Effect of Bupi Qiangli Compound on mRNA Expression Level of Glucocorticoid Receptor in Thymus of EAMG Model Rats

作　　者：姜雄[1] 何前松[2] 况时祥[2] 

机构地区：[1]贵阳中医学院研究生院,贵州贵阳550002 [2]贵阳中医学院第二附属医院,贵州贵阳550003

出　　处：《辽宁中医杂志》2017年第2期398-402,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81360605)

摘　　要：目的:观察补脾强力复方对实验性自身免疫性重症肌无力(EAMG)模型大鼠胸腺组织中糖皮质激素受体(GR)mRNA表达的影响。方法:将Lewis大鼠按体重随机分为6组,即:正常组、模型组、强的松组(5.4 mg/kg)、补脾强力复方不同剂量组(剂量分别为4.75 g/kg,7.12 g/kg,9.49 g/kg),除正常组外,均采用鼠源性AchR-α亚基97-116肽段序列(R97-116)免疫接种复制EAMG模型。药物治疗后,ELISA法检测各组大鼠的AchR-Ab、皮质醇水平,RT-Q-PCR技术检测大鼠胸腺组织GR mRNA表达水平。结果:与正常组比较,模型组GR mRNA相对表达量降低(P<0.01),与模型组比较,西药组、中药大、中、小剂量组大鼠AchR-Ab含量下降(P<0.05),强的松组、中药大剂量组和中剂量组GR mRNA相对表达量增加(P<0.05)。结论:补脾强力复方可提高胸腺组织GR mRNA的表达,这可能是其治疗MG的作用机制之一。Objective ： To observe the effect of Bupi Qiangli Compound on the mRNA expression level of glucocorticoid receptor （GR） in the thymus of experimental autoimmune myasthenia gravis（EAMG） model rats. Methods ： Female Lewis rats were random- ly divided into six groups according to the different weight. That is：normal group, model group, Prednisone group （5.4 mg/kg）, different dose of Bupi Qiangli Compound groups（4.75 g/kg,7.12 g/kg,9.49 g/kg）. All Lewis rats were immunized with 97 - 116 peptide of the rat original AchR α- subunit（ R97 -116） to copy EAMG rats model except for normal group. The levels of AchR - Ab and cortisol were determined by ELISA and the mRNA expression levels of GR were determined by RT - Q - PCR af- ter the treatment. Results ： Compared to the normal group, the mRNA expression levels of GR decreased in model group （ P 〈 0. 01 ）. Compared to the model group, the levels of AchR - Ab decreased in Prednisone group and different dose of Bupi Qiangli compound groups（P 〈 0.05 ）,the mRNA expression levels of GR increased in Prednisone group,Bupi Qiangli compound large and middle dose treatment group （P 〈 0.05 ）. Conclusion：Bupi Qiangli Compound can increase the mRNA expression level of glu- cocorticoid receptor in thymus, which may be one of the therapeutic mechanism of MG.

关 键 词：补脾强力复方 EAMG 糖皮质激素受体 

分 类 号：R285[医药卫生—中药学]