与小麦抗白粉病基因Pm48紧密连锁分子标记的开发  

Development of Markers Closely Linked with Wheat Powdery Mildew Resistance Gene Pm48

作　　者：付必胜[1] 刘颖[1,2] 张巧凤[1] 吴小有[1] 高海东 蔡士宾[1] 戴廷波[2] 吴纪中[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省农业种质资源保护与利用平台,江苏南京210014 [2]南京农业大学农学院,江苏南京210095 [3]南京集思慧远生物科技有限公司,江苏南京210014

出　　处：《作物学报》2017年第2期307-312,共6页Acta Agronomica Sinica

基　　金：国家科技支撑计划项目(2013BAD01B02-12);国家现代农业产业技术体系建设专项(CARS-3-1-17);江苏省农业科技自主创新资金项目(CX(14)5006);江苏省自然科学基金(BK2012783)资助~~

摘　　要：Pm48为本实验室鉴定的一个抗白粉病新基因。为精细定位该基因,利用混池ddRAD测序鉴定了81个与该基因关联的序列,开发了STS标记Xmp931,转化了CAPS标记Xmp928、Xmp930和Xmp936;同时,利用粗山羊草基因组序列开发了71个基因组SSR标记,定位了其中的Xmp1089和Xmp1112。在115个宁糯麦1号′Tabasco衍生的F2:3家系中,Xmp928与目的基因共分离,Xmp1112位于近着丝粒方向处距抗病基因3.1 c M。在671个纯合感病家系中,标记Xmp928仍与目的基因共分离。利用3个中国春5DS缺失系,最终将Pm48定位在小麦5DS上0.63–0.67的臂区段中。Pm48 is a novel powdery mildew resistance gene identified previously in our laboratory. This study aimed at developing close molecular markers for fine mapping of the gene. The ddRAD-sequencing assay revealed 81 SNPs associated with the target gene, in which one converted into the STS marker Xmp931 and three converted into the CAPS markers Xmp928, Xmp930, and Xmp936. We also developed 71 genomic SSR markers according to the genome sequence of Aegilops tauschii. And mapped two of them, Xmp1089 and Xmp1112. Using the 115 F2：3 families derived from the cross of Ningnuomai 1 ′ Tabasco, the target gene was found to be co-segregated with Xmp928 and distal to Xmp1112 with the genetic distance of 3.1 c M towards centromere. In the 671 homozygous susceptible families, Xmp928 also showed co-segregated with the target gene. We also physically mapped Pm48 to the bin of 5DS 0.63–0.67 by using three Chinese Spring 5DS deletion lines.

关 键 词：小麦 抗白粉病基因 分子标记 混池ddRAD测序 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]